Evans L A, Sheppard M, May J T
Arch Virol. 1985;85(1-2):13-23. doi: 10.1007/BF01317002.
Genital herpes simplex virus type 2 (HSV-2) infections can be distinguished from present or past HSV-1 infections by an AG-4 antigen complement fixation assay. The assay which utilizes a 4 hour HSV-2 infected cell extract prepared at a multiplicity of infection (MOI) of 1.0 PFU/cell, appears to consist of several viral proteins. Studies using monoclonal antibodies, polyclonal rabbit hyperimmune serum, HSV-1 X HSV-2 intertypic recombinant viruses and polyacrylamide gel electrophoresis suggest that ICP 8 may be one of the major antigens involved in the complement fixing reaction. It is probable that the success of the assay is not due to a true type specificity but rather a threshold phenomenon in which HSV-2 extracts contain more early viral antigens (including ICP 8) and sera from HSV-2 patients contain more complement fixing antibody to these antigens.
通过AG - 4抗原补体结合试验可将2型单纯疱疹病毒（HSV - 2）感染与现有的或既往的HSV - 1感染区分开来。该试验利用在感染复数（MOI）为1.0 PFU/细胞的条件下制备的4小时HSV - 2感染细胞提取物，该提取物似乎由几种病毒蛋白组成。使用单克隆抗体、多克隆兔超免疫血清、HSV - 1×HSV - 2型间重组病毒以及聚丙烯酰胺凝胶电泳进行的研究表明，ICP 8可能是参与补体结合反应的主要抗原之一。该试验的成功可能并非由于真正的型特异性，而是一种阈值现象，即HSV - 2提取物含有更多早期病毒抗原（包括ICP 8），且HSV - 2患者的血清含有更多针对这些抗原的补体结合抗体。
