Friedmann T, LaPorte P, Esty A
J Biol Chem. 1978 Sep 25;253(18):6561-7.
The nucleotide sequence of polyoma DNA, from near the Hpa II 3/5 unction to the Hpa II 4/ae III 18 junction has been determined by the chemical method of Maxam and Gilbert (Maxam, A., and Gilbert, W. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 560--564). The sequence contains 878 base paris, including the origin of DNA replication and the region known to encode the hr-t function. The region corresponding to the origin of DNA replication contains several short-repeated sequences and palindromes. There is a 30-base-pair region with striking similarity to the corresponding region of SV40, and, as in SV40, a portion of that sequence is capable of forming a stable hairpin loop. In the region encoding the hr-t function, there is apparently a single open reading frame extending from position 188 to theHpa III 4/Hae III 18 junction. The potential translation product of this open frame begins with an initiation codon starting at position 188, and the first five amino acids of this product are Met-Asp-Arg-Val-Leu. This sequence is similar to the NH2-terminal five amino acids of SV40 small t-antigen known from nucleotide and amino acid sequencing to be Met-Asp-Lys-Val-Leu.
多瘤病毒DNA从Hpa II 3/5连接处附近到Hpa II 4/Hae III 18连接处的核苷酸序列已通过Maxam和Gilbert的化学方法（Maxam, A., and Gilbert, W. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 560--564）测定。该序列包含878个碱基对，包括DNA复制起点和已知编码hr-t功能的区域。对应于DNA复制起点的区域包含几个短重复序列和回文序列。有一个30个碱基对的区域与SV40的相应区域有显著相似性，并且与SV40一样，该序列的一部分能够形成稳定的发夹环。在编码hr-t功能的区域，显然有一个单一的开放阅读框，从第188位延伸到Hpa III 4/Hae III 18连接处。这个开放阅读框的潜在翻译产物从第188位的起始密码子开始，该产物的前五个氨基酸是Met-Asp-Arg-Val-Leu。这个序列与SV40小t抗原的氨基末端五个氨基酸相似，从核苷酸和氨基酸测序可知为Met-Asp-Lys-Val-Leu。
