牛促肾上腺皮质激素-β-促脂解素前体基因区域重复DNA序列的结构分析
Structural analysis of repetitive DNA sequences in the bovine corticotropin-beta-lipotropin precursor gene region.
作者信息
Watanabe Y, Tsukada T, Notake M, Nakanishi S, Numa S
出版信息
Nucleic Acids Res. 1982 Mar 11;10(5):1459-69. doi: 10.1093/nar/10.5.1459.
Abstract
Repetitive DNA sequences in the bovine corticotropin-beta-lipotropin precursor gene region have been mapped and subjected to nucleotide sequence analysis. Two of the four repetitive DNA segments found are located in the 5'-flanking region, and one each within the intervening sequences. Each repetitive DNA segment contains one to three highly homologous unit sequences with an approximate length of 120 base pairs. All the unit sequences are flanked on the 3' side by tandem repeats. There are about 10(5) copies of the repetitive DNA in the bovine genome. Comparison of the bovine repetitive sequences with those of other mammalian species reveals the presence of a homologous segment of approximately 40 base pairs. This segment and the region preceding it in the bovine repetitive DNA exhibit sequence homology with the region encompassing the origin of DNA replication in papovaviruses.
摘要
牛促肾上腺皮质激素-β-促脂素前体基因区域中的重复DNA序列已被定位并进行了核苷酸序列分析。所发现的四个重复DNA片段中的两个位于5'侧翼区域，另外两个分别位于间隔序列中。每个重复DNA片段包含一到三个高度同源的单位序列，长度约为120个碱基对。所有单位序列在3'侧均由串联重复序列侧翼。牛基因组中约有10⁵个重复DNA拷贝。将牛的重复序列与其他哺乳动物物种的重复序列进行比较，发现存在一个约40个碱基对的同源片段。该片段及其在牛重复DNA中之前的区域与乳头瘤病毒中包含DNA复制起点的区域具有序列同源性。
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