Vu H, McCollum C, Lotys C, Andrus A
Applied Biosystems, Foster City, CA 94404.
Nucleic Acids Symp Ser. 1990(22):63-4.
The optimal system for the rapid, efficient, convenient, and economical synthesis and purification of synthetic oligonucleotides has been advancing. By recognizing the very rapid reaction kinetics and taking advantage of an efficient, low volume delivery system, cycle times have decreased to about 5.5 minutes, without compromising synthesis performance. A new set of base protecting groups for cyanoethylphosphoramidite nucleoside monomers have been developed, which decreases the post-synthesis time requirements. A particular form of polystyrene has also been developed as a solid support for automated oligonucleotide synthesis. Typical sequencing or PCR primers (20mers) now require less than 2 hours for synthesis and 2 hours for cleavage and deprotection.
用于快速、高效、便捷且经济地合成与纯化合成寡核苷酸的优化系统一直在不断发展。通过认识到非常快速的反应动力学并利用高效、小体积的输送系统，循环时间已缩短至约5.5分钟，而不影响合成性能。已开发出一套用于氰乙基亚磷酰胺核苷单体的新型碱基保护基团，这减少了合成后所需的时间。还开发了一种特殊形式的聚苯乙烯作为自动化寡核苷酸合成的固相载体。典型的测序或PCR引物（20聚体）现在合成所需时间不到2小时，切割和脱保护所需时间为2小时。
