Steven F S, Maier H, Born I A, Altmannsberger M
Dep. of Biochemistry and Molecular Biology, Univ. of Manchester.
Dtsch Z Mund Kiefer Gesichtschir. 1990 Jan-Feb;14(1):32-5.
A novel fluorescence microscopic technique, based on the application of low-molecular fluorescent dyes, was used to study the expression of the enzyme guanidinobenzoatase in malignant tumors of the head and neck area. Frozen sections have shown that moderately and highly differentiated squamous cell carcinomas express this enzyme, particularly in the area of tumor invasion. The protease is capable of breaking down fibronectin, an important component of the extracellular matrix. In less differentiated squamous cell carcinomas as well as malignant melanomas almost all tumor cells exhibited guanidinobenzoatase activity. The fluorescence microscopic technique presented here provides a fast and easy method for the localization of tumor cells. At the same time it provides a better understanding of the biological control of a tumor-associated protease, thus furnishing important new information on the invasion behavior of malignant tumors.
一种基于低分子荧光染料应用的新型荧光显微镜技术，被用于研究头颈区域恶性肿瘤中胍基苯甲酸酶的表达。冰冻切片显示，中高分化的鳞状细胞癌表达这种酶，尤其在肿瘤浸润区域。该蛋白酶能够分解细胞外基质的重要成分纤连蛋白。在低分化鳞状细胞癌以及恶性黑色素瘤中，几乎所有肿瘤细胞都表现出胍基苯甲酸酶活性。这里介绍的荧光显微镜技术为肿瘤细胞的定位提供了一种快速简便的方法。同时，它能更好地理解肿瘤相关蛋白酶的生物学调控，从而为恶性肿瘤的侵袭行为提供重要的新信息。
