Heinrich H W
Friedrich-Loeffler-Institut Insel Riems.
Arch Exp Veterinarmed. 1990;44(6):849-53.
Several DNA viruses have recently emerged as useful eucaryotic vectors. The ability to incorporate large amounts of foreign DNA in the vaccinia virus genome without loss of infectivity, evidence to correct glycosylation and processing of expressed proteins and the wide host range of vaccinia virus all contribute to the versatility of this system as a research tool. The development of recombinant DNA technology and its use in genetic engineering has provided opportunities to construct DNA-recombinant viruses that can provide protection against a spectrum of diseases. A brief account is given of current developments and future prospects of DNA recombinant viruses, with particular reference to vaccinia virus.
最近，几种DNA病毒已成为有用的真核载体。痘苗病毒基因组能够整合大量外源DNA而不丧失感染力，能够正确糖基化和加工表达蛋白，且宿主范围广泛，这些特性共同促成了该系统作为研究工具的多功能性。重组DNA技术的发展及其在基因工程中的应用为构建可抵御一系列疾病的DNA重组病毒提供了契机。本文简要介绍了DNA重组病毒的当前进展和未来前景，尤其侧重于痘苗病毒。
