Jones L, Tenorio E, Gorham J, Yilma T
Department of Veterinary Pathology, University of California, Davis 95616, USA.
Am J Vet Res. 1997 Jun;58(6):590-3.
To investigate the ability of rinderpest virus (RPV) antigens, expressed in pox virus vectors, to protect against canine distemper virus (CDV) infection in ferrets.
Ferrets (Mustela putorius; n = 27) with no previous exposure to CDV.
Ferrets were inoculated intradermally with recombinant vaccinia viruses expressing the H gene of RPV, the F gene of RPV, the H and F genes of RPV, or fowlpox virus recombinant expressing both genes. Two ferrets were vaccinated s.c. with CDV vaccine as positive controls, and 1 group was left unvaccinated as a negative control. Blood was obtained from ferrets biweekly; antibody titer to RPV was detected by ELISA, and CDV antibody titer was measured by serum neutralization testing and ELISA.
Partial protection was seen in all groups, with vRVFH vaccination being the most protective (60%).
A single inoculation with a vaccinia virus expressing the H and F genes of RPV was able to protect 60% of the vaccinated ferrets challenge exposed with a high dose of CDV. These results indicate the ability of RPV antigens expressed by vaccinia virus to protect ferrets against a related morbillivirus. Further, they document the safety and efficacy of a recombinant vaccinia virus vaccine for ferrets. Such vaccines may be useful given the susceptibility of ferrets to CDV and the problem of maternal antibody interfering with vaccination of young animals.
研究痘病毒载体表达的牛瘟病毒（RPV）抗原对雪貂抵抗犬瘟热病毒（CDV）感染的保护能力。
未曾接触过CDV的雪貂（白鼬；n = 27）。
给雪貂皮内接种表达RPV的H基因、RPV的F基因、RPV的H和F基因的重组痘苗病毒，或表达这两个基因的禽痘病毒重组体。两只雪貂皮下接种CDV疫苗作为阳性对照，一组不接种作为阴性对照。每两周从雪貂采集血液；通过ELISA检测针对RPV的抗体效价，通过血清中和试验和ELISA测定CDV抗体效价。
所有组均观察到部分保护作用，接种vRVFH的保护作用最强（60%）。
单次接种表达RPV的H和F基因的痘苗病毒能够保护60%的接种雪貂免受高剂量CDV攻击。这些结果表明痘苗病毒表达的RPV抗原能够保护雪貂抵抗相关的麻疹病毒。此外，它们证明了重组痘苗病毒疫苗对雪貂的安全性和有效性。鉴于雪貂对CDV易感以及母源抗体干扰幼龄动物疫苗接种的问题，此类疫苗可能会有用。
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