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Rapid microwave fixation of rat brain.

作者信息

Moroji T, Takahashi K, Ogura K, Toishi T, Arai S

出版信息

J Microw Power. 1977 Dec;12(4):273-86. doi: 10.1080/16070658.1977.11689055.

DOI:10.1080/16070658.1977.11689055
PMID:210282
Abstract

A new microwave device which inactivates brain enzymes rapidly and uniformly is described. From the results obtained with microwave irradiation at two power levels (0.8 kW and 4.5 kW), it has been demonstrated that the high power microwave irradiation has several advantages over the low power irradiation. In its application to neurochemical studies, significant increase in the DOPAC level was found in the irradiated brain, while there were no statistical differences in the levels of NE, DA, 5-HT and 5-HIAA between microwave irradiation and decapitation. Significant increase in the Ach level and marked reduction of the choline level were observed after microwave irradiation. There were no significant differences in the level of cyclic GMP in the brain between the two methods of sacrifice, while significant reduction of the cyclic AMP level was observed in the irradiated brain.

摘要

相似文献

1
Rapid microwave fixation of rat brain.
J Microw Power. 1977 Dec;12(4):273-86. doi: 10.1080/16070658.1977.11689055.
2
[Levels of biogenic amines and their metabolites in rat whole brain after rapid tissue fixation with microwave irradiation (author's transl)].微波辐照快速组织固定后大鼠全脑中生物胺及其代谢产物的水平(作者译)
No To Shinkei. 1978 Dec;30(12):1303-8.
3
A new microwave device for rapid thermal fixation of the murine brain.
J Microw Power. 1978 Mar;13(1):53-7. doi: 10.1080/16070658.1978.11689075.
4
The tissue content of cyclic AMP in rats after microwave irradiation in vivo.大鼠体内微波辐射后环磷酸腺苷的组织含量
J Pharmacobiodyn. 1980 May;3(5):230-5. doi: 10.1248/bpb1978.3.230.
5
Fast fixation of brain in situ by high intensity microwave irradiation: application to neurochemical studies.
J Microw Power. 1976 Mar;11(1):61-5. doi: 10.1080/00222739.1976.11688987.
6
Regional levels of cyclic AMP in rat brain: pitfalls of microwave inactivation.大鼠脑中环磷酸腺苷的区域水平:微波灭活的陷阱
J Cyclic Nucleotide Res. 1977 Oct;3(5):367-79.
7
Cyclic AMP in the rat brain: microwave irradiation as a means of tissue fixation.大鼠脑中的环磷酸腺苷:微波辐射作为一种组织固定方法
Adv Cyclic Nucleotide Res. 1972;1:425-34.
8
Cyclic GMP and cyclic AMP levels in median eminence, hypothalamus and pituitary gland of the rat after decapitation or microwave irradiation.
Neurosci Lett. 1979 Jul;13(2):189-94. doi: 10.1016/0304-3940(79)90040-5.
9
Cyclic adenosine monphosphate, metabolites, and phosphorylase in neural tissue: a comparison a methods of fixation.神经组织中的环磷酸腺苷、代谢产物和磷酸化酶:固定方法的比较
Science. 1973 Jul 20;181(4096):280-2. doi: 10.1126/science.181.4096.280.
10
Cyclic adenosine monophosphate in brain areas: microwave irradiation as a means of tissue fixation.脑区中的环磷酸腺苷:微波辐射作为一种组织固定手段
Science. 1971 Sep 17;173(4002):1142-3. doi: 10.1126/science.173.4002.1142.

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