Vorst O, van Dam F, Oosterhoff-Teertstra R, Smeekens S, Weisbeek P
Department of Molecular Cell Biology, University of Utrecht, Netherlands.
Plant Mol Biol. 1990 Apr;14(4):491-9. doi: 10.1007/BF00027495.
We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tissue-specific and positively affected by light. Response times, both to dark and light conditions, are remarkably rapid. A chimeric gene consisting of a 1.2 kb Fd promoter fragment fused to the beta-glucuronidase reporter gene was transferred to tobacco. This fusion gene is expressed in a tissue-specific way; it shows high levels of expression in green leaves, as compared to root tissue.
我们从拟南芥中分离并分析了一个ferredoxin基因。该基因编码一个148个氨基酸的前体蛋白，其中包括一个52个残基的叶绿体转运肽。Southern分析表明，在拟南芥基因组中存在该铁氧化还原蛋白（Fd）基因的单拷贝。其表达具有组织特异性，并受到光照的正向影响。对黑暗和光照条件的响应时间都非常迅速。一个由1.2 kb Fd启动子片段与β-葡萄糖醛酸酶报告基因融合而成的嵌合基因被转入烟草。这个融合基因以组织特异性的方式表达；与根组织相比，它在绿叶中表现出高水平的表达。
