Schneider M, Ow D W, Howell S H
Boyce Thompson Institute, Cornell University, Ithaca, NY 14853-1801.
Plant Mol Biol. 1990 Jun;14(6):935-47. doi: 10.1007/BF00019391.
Expression of the firefly luciferase gene in transgenic plants produces light emission patterns when the plants are supplied with luciferin. We explored whether in in vivo pattern of light emission truly reveals the pattern of luciferase gene expression or whether it reflects other parameters such as the availability of the substrate, luciferin, or the tissue-specific distribution of organelles in which luciferase was localized. The tissue-specific distribution of luciferase activity and the in vivo pattern of light were examined when the luciferase gene was driven by different promoters and when luciferase was was redirected from the peroxisome, where it is normally targeted, to the chloroplast compartment. It was found that the distribution of luciferase activity closely correlated with the tissue-specific pattern of luciferase mRNA. However, the in vivo light pattern appeared to reflect not only tissue-specific distribution of luciferase activity, but also the pattern of luciferin uptake.
当向植物提供荧光素时，萤火虫荧光素酶基因在转基因植物中的表达会产生发光模式。我们探究了体内发光模式是真的揭示了荧光素酶基因的表达模式，还是反映了其他参数，如底物荧光素的可用性，或荧光素酶所在细胞器的组织特异性分布。当荧光素酶基因由不同启动子驱动时，以及当荧光素酶从其正常定位的过氧化物酶体重新定位到叶绿体区室时，检测了荧光素酶活性的组织特异性分布和体内发光模式。结果发现，荧光素酶活性的分布与荧光素酶mRNA的组织特异性模式密切相关。然而，体内发光模式似乎不仅反映了荧光素酶活性的组织特异性分布，还反映了荧光素摄取模式。
