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通过M13mp7鸟枪法测序获得的花椰菜花叶病毒感染性克隆的完整核苷酸序列。

The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing.

作者信息

Gardner R C, Howarth A J, Hahn P, Brown-Luedi M, Shepherd R J, Messing J

出版信息

Nucleic Acids Res. 1981 Jun 25;9(12):2871-88. doi: 10.1093/nar/9.12.2871.

Abstract

We have determined the complete primary structure (8031 base pairs) of an infectious clone of cauliflower mosaic virus strain CM1841. The sequence was obtained using the strategy of cloning shotgun restriction fragments in the sequencing vector M13mp7. Comparison of the CM1841 sequence with that published for another caMV strain (Strasbourg) reveals 4.4% changes, mostly nucleotide substitutions with a few small insertions and deletions. The six open reading frames in the sequence of the Strasbourg isolate are also present in CM1841.

摘要

我们已经确定了花椰菜花叶病毒CM1841株感染性克隆的完整一级结构(8031个碱基对)。该序列是通过将鸟枪法限制性片段克隆到测序载体M13mp7中的策略获得的。将CM1841序列与已发表的另一种花椰菜花叶病毒株(斯特拉斯堡株)的序列进行比较,发现有4.4%的变化,主要是核苷酸替换,还有一些小的插入和缺失。斯特拉斯堡分离株序列中的六个开放阅读框在CM1841中也存在。

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