Department of Chemistry and CREOL, The College of Optics and Photonics, University of Central Florida, 4000 Central Florida Parkway, Orlando, Florida 32816, USA.
ACS Appl Mater Interfaces. 2010 Nov;2(11):2978-81. doi: 10.1021/am100719v. Epub 2010 Oct 28.
A hydrophilic bis(1,2,3-triazolyl)fluorene derivative was synthesized as a multi-photon-absorbing, zinc-ion-sensing fluorescent probe. The fluorescence response was approximately five-fold greater in presence of Zn(2+), resulting in a large binding constant (1 × 10(9)) for a 1:2 ligand to zinc complex. A four-fold increase in the two-photon absorption cross section was achieved upon binding Zn(2+). In vitro two-photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn(2+) into HeLa cells and reversible Zn(2+) binding, demonstrating the potential of this probe for zinc ion sensing.
合成了一种亲水双（1,2,3-三唑基）芴衍生物，用作多光子吸收、锌离子传感荧光探针。在存在 Zn(2+)的情况下，荧光响应大约增加了五倍，导致配体与锌络合物的结合常数（1×10(9))较大。结合 Zn(2+)后，双光子吸收截面增加了四倍。体外双光子荧光显微镜成像表明，将 Zn(2+)引入 HeLa 细胞后，荧光显著增加，并且锌离子可以可逆结合，这表明该探针在锌离子传感方面具有应用潜力。
