Chen Xiao-Yun, Shi Jing, Li Yi-Ming, Wang Feng-Liang, Wu Xu, Guo Qing-Xiang, Liu Lei
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Org Lett. 2009 Oct 1;11(19):4426-9. doi: 10.1021/ol901787w.
A new fluorescent probe for monitoring Zn(2+) was synthesized based on the structure of 7-hydroxyquinoline. Compared with 8-substituted quinolines, the new probe exhibited higher selectivity for Zn(2+) over Cd(2+). Its fluorescence enhancement (14-fold) and nanomolar range sensitivity (K(d) = 0.117 nM) were favorable toward biological applications. Experiments also showed that a cell-permeable derivative of the new probe was potentially useful for two-photon imaging in living cells.
基于7-羟基喹啉的结构合成了一种用于监测Zn(2+)的新型荧光探针。与8-取代喹啉相比，该新型探针对Zn(2+)的选择性高于Cd(2+)。其荧光增强（14倍）和纳摩尔范围的灵敏度（K(d)=0.117 nM）有利于生物应用。实验还表明，该新型探针的细胞可渗透衍生物在活细胞的双光子成像中具有潜在用途。
