Universidade Federal do Rio de Janeiro (UFRJ), Universidade Estadual do Rio de Janeiro (UERJ), Rio de Janeiro, RJ, Brazil.
Lett Appl Microbiol. 2010 Dec;51(6):611-8. doi: 10.1111/j.1472-765X.2010.02946.x. Epub 2010 Oct 4.
To evaluate an integrated aquaculture system, microbiological analyses of water used in this system were carried out and the incidence and antimicrobial resistance of enteropathogens were determined in the related ecosystem.
Microbiological analysis was undertaken for Salmonella sp., Shigella sp., Vibrio sp. and Aeromonas sp. The disc-diffusion method was performed according to the Clinical and Laboratory Standards Institute. Water samples tested had 32.9% of faecal coliform rates (≤1600 per 100 ml) in accordance with WHO for pisciculture in wastewater. Salmonella spp. were detected in 14.5% of the samples. From a total of 33 strains, 15.1% were resistant to one or two antimicrobial drugs tested and multidrug resistance was not observed. Aeromonas spp. were identified in 91.6% of the samples. From a total of 416 strains, resistance to one antimicrobial class was observed in 66.3% and multidrug resistance in 37.7%.
This system reflects the community profile, drawing attention to the circulation of pathogens, because the genes coding for resistance to classical antibiotics and broad spectrum are a public health problem.
The reuse of water resources requires continuous monitoring as the system is subject to treatment failure, which can result in the spread of bacterial pathogens.
评估水产养殖综合系统，对该系统使用的水进行微生物分析，并确定相关生态系统中肠道病原体的发病率和抗药性。
对沙门氏菌、志贺氏菌、弧菌和气单胞菌进行微生物分析。采用临床和实验室标准协会的圆盘扩散法进行检测。水样的粪便大肠菌群率符合世卫组织规定的废水水产养殖标准，为 32.9%（每 100 毫升≤1600 个）。在总共 33 株菌中，有 15.1%对一种或两种测试的抗菌药物有耐药性，没有观察到多药耐药性。在 91.6%的水样中鉴定出气单胞菌。在总共 416 株菌中，有 66.3%对一种抗菌药物类别有耐药性，37.7%对多种药物有耐药性。
该系统反映了群落特征，引起了人们对病原体循环的关注，因为编码对经典抗生素和广谱抗生素耐药性的基因是一个公共卫生问题。
水资源的再利用需要持续监测，因为该系统可能会出现处理失败的情况，从而导致细菌病原体的传播。
