Liu Shu-hua, Xie Wei-guo, Kremer Mathias, Machens Hans Guenther, Lankenau Eva Maria, Huettmann Gereon
Institute of Burns, Wuhan City Hospital NO.3 & Tongren Hospital of Wuhan University, Wuhan 430060, China.
Zhonghua Shao Shang Za Zhi. 2010 Aug;26(4):272-5.
To evaluate the application value of Optical Coherence Tomography (OCT) in the diagnosis of the depth of burn wound.
Deep partial-thickness scald models of Skh-1 mice were reproduced using self-made steam scald appliance. The scald wounds were scanned with OCT 3 hours, or 3 and 8 days after injury respectively. Scanned wound tissue was harvested for histological examination right after each episode of OCT imaging. Normal skin of mice was scanned and examined with the above-mentioned methods at the same time.
Compared with those of the normal skin, collagen in the dermis was denatured after steam scald, and it was imaged as vanishing or reduction in birefringence in OCT detection. The structure change intensity was related to the pathological process of the wounds and consistent with the corresponding histological results.
OCT is a noninvasive technique. It can be used to diagnose the depth of burn wound in real time.
评估光学相干断层扫描（OCT）在烧伤创面深度诊断中的应用价值。
使用自制蒸汽烫伤装置建立Skh-1小鼠深Ⅱ度烫伤模型。分别在伤后3小时、3天和8天用OCT对烫伤创面进行扫描。每次OCT成像后立即采集扫描的创面组织进行组织学检查。同时用上述方法对小鼠正常皮肤进行扫描和检查。
与正常皮肤相比，蒸汽烫伤后真皮中的胶原蛋白发生变性，在OCT检测中表现为双折射消失或减弱。结构变化强度与创面病理过程相关，且与相应的组织学结果一致。
OCT是一种无创技术。它可用于实时诊断烧伤创面深度。
