Ben-Eliahu Shmuel, Tal Kfir, Milstein Asher, Levin-Harrus Tal, Ezov Nati, Kleinmann Guy
Harlan Biotech Israel Ltd, Rehovot, Israel.
Ophthalmic Surg Lasers Imaging. 2011 Mar-Apr;42(2):152-6. doi: 10.3928/15428877-20110125-01. Epub 2011 Feb 1.
To evaluate the protective effect of different ophthalmic viscosurgical devices (OVDs) on corneal endothelial cells against relatively severe phacoemulsification damage in a rabbit model.
Twenty-four rabbit eyes were randomly assigned to four similar groups: in three groups the aqueous humor was completely replaced by Visiol (TRB CHEMEDICA, München, Germany), Biolon (Bio-Technology General Ltd., Kiryat Malachi, Israel), and Viscoat (Alcon, Puurs, Belgium) and in the control group no OVD was applied. Endothelial cell counts were performed prior to initiating the study. All eyes were exposed to continuous 5 minutes of phacoemulsification. Endothelial cell counts were repeated 4 days postoperatively.
Viscoat showed the highest endothelial cell loss (30%), followed by Biolon (25%), Visiol (22%), and the control group (19%). None of the differences between the groups were found to be statistically significant, although they were within each group (P = .028).
None of the tested OVDs demonstrated protective effect on corneal endothelial cells in comparison to the control group. This model was found to be too aggressive for the demonstration of the protective effect of different OVDs even for hard cataract.
在兔模型中评估不同眼科粘弹剂（OVDs）对角膜内皮细胞抵御相对严重的超声乳化损伤的保护作用。
将24只兔眼随机分为四组，每组情况相似：三组中房水分别用德国慕尼黑TRB CHEMEDICA公司的Visiol、以色列基里亚特拉马奇的生物技术通用有限公司的Biolon以及比利时普尔阿尔康公司的Viscoat完全替代，对照组不使用OVD。在研究开始前进行内皮细胞计数。所有眼睛均接受连续5分钟的超声乳化。术后4天重复进行内皮细胞计数。
Viscoat组内皮细胞损失率最高（30%），其次是Biolon组（25%）、Visiol组（22%）和对照组（19%）。尽管每组内存在差异（P = 0.028），但各组之间的差异均无统计学意义。
与对照组相比，所测试的OVDs均未对角膜内皮细胞显示出保护作用。发现该模型即使对于硬核白内障，对于展示不同OVDs的保护作用也过于激进。
