Department of Environment and Agro-biotechnologies, CRP-Gabriel Lippmann, 41 rue du Brill, L-4422 Belvaux, GD Luxembourg.
Tree Physiol. 2010 Nov;30(11):1415-32. doi: 10.1093/treephys/tpq082.
Populus tremula L. × Populus alba L. (Populus ×c anescens (Aiton) Smith) - clone INRA 717-1-B4 saplings (50 cm apex to base and carrying 19 leaves on average) - were followed for 28 days. Half of the trees were grown in charcoal-filtered air while the other half were exposed to 120 ppb ozone for 11 h a day during the light period. The expanding leaf number 4 was tagged at the beginning of the experiment and finished expansion between 7 and 14 days. These leaves were harvested weekly for biochemical and proteome analyses using quantitative bidimensional electrophoresis (DiGE). Independent of the ozone treatment, all the analyses allowed a distinction between expanding and adult leaves. The results indicate that during the expansion phase (Days 0-7) the enzymatic machinery of the leaves is set up, and remains dynamically stable in the adult leaves (Days 14-28). Although ozone had no apparent effect on expanding leaves, the metabolic stability in fully expanded leaves observed in ozone-free plants was disturbed after 2 weeks of exposure and a stress-induced response became apparent.
颤杨×银白杨（杨属×坎塞雷斯（艾顿）·史密斯）无性系 INRA 717-1-B4 实生苗（顶梢到根基长 50 厘米，平均带有 19 片叶子）被监测了 28 天。一半的树木生长在经过木炭过滤的空气中，而另一半则在光照期间每天暴露在 120ppb 臭氧中 11 小时。在实验开始时，将第 4 片展开的叶子贴上标签，在第 7 天到第 14 天之间完成展开。这些叶子每周用于生化和蛋白质组分析，使用定量二维电泳（DiGE）。无论臭氧处理如何，所有分析都可以区分展开叶和成年叶。结果表明，在展开阶段（第 0-7 天），叶子的酶机制已经建立，并在成年叶子中保持动态稳定（第 14-28 天）。尽管臭氧对展开的叶子没有明显影响，但在无臭氧植物中观察到的完全展开叶子中的代谢稳定性在暴露 2 周后受到干扰，出现了应激诱导反应。
