Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Chongqing Medical University, Chongqing, People's Republic of China.
Nanotechnology. 2010 Nov 26;21(47):475104. doi: 10.1088/0957-4484/21/47/475104. Epub 2010 Oct 29.
Near-infrared (near-ir) quantum dots (QDs) are well known for their excellent optical characteristics. They hold great potential for applications in non-invasive long term observation and tracing of cells in vivo. Here, near-ir QDs with an emission wavelength of 800 nm (QD800) were used to label squamous cell carcinoma cell line U14 (U14/QD800). The effect of tissue depth and animal fur on the imaging sensitivity and stability was evaluated following subcutaneous and intramuscular injection into Kunming mice, employing an in vivo imaging system. We have demonstrated that QD800-based visual in vivo imaging increased the sensitivity of cancer early detection by a factor of 100 compared with traditional detection methods. More importantly, this study proved for the first time that animal fur has a serious impact on the detection sensitivity and duration of QD-based in vivo imaging. In general, the duration and sensitivity of QD800 for in vivo imaging were not greatly affected by a depth less than 1.8 ± 0.21 mm (subcutaneous or intramuscular). This study provides critical reference data for further research on near-ir QD-based early detection and in vivo visual observation of cancer.
近红外（近红外）量子点（QD）以其优异的光学特性而闻名。它们在应用于非侵入性的长期观察和体内细胞的追踪方面具有巨大的潜力。在这里，使用发射波长为 800nm（QD800）的近红外 QD 标记鳞状细胞癌细胞系 U14（U14/QD800）。通过活体成像系统，评估了皮下和肌肉内注射到昆明小鼠后组织深度和动物皮毛对成像灵敏度和稳定性的影响。我们已经证明，与传统检测方法相比，基于 QD800 的视觉活体成像将癌症早期检测的灵敏度提高了 100 倍。更重要的是，本研究首次证明动物皮毛对基于 QD 的活体成像的检测灵敏度和持续时间有严重影响。一般来说，QD800 进行活体成像的持续时间和灵敏度在深度小于 1.8±0.21mm（皮下或肌肉内）时不会受到很大影响。本研究为近红外 QD 基于早期检测和癌症的活体可视化观察的进一步研究提供了关键的参考数据。
