Yang Kai, Cao Yu-an, Li Zhi-gang, Lü Xiao-qiang, Zhao Cheng, Yang Jia
Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2011 Mar;46(3):134-8.
To examine the in vivo visual imaging of buccal carcinoma with the near-infrared fluorescent quantum dots.
The U14 cells were labeled by endocytosis with QD800 (U14/QD800) which was linked with cell-penetrating peptide. Different number of U14/QD800 was injected under the buccal mucosa of nude mice and Kunming mice separately and imaged at different time to detect the in vivo sensitivity and dynamic imaging of U14/QD800.
The minimum number of U14/QD800 cells which could be detected by in vivo imaging system was 1 × 10(4) in nude mice's cheek and 1 × 10(5) in Kunming mice's. The time for visual imaging of 1 × 10(4), 1 × 10(5) and 1 × 10(6) U14/QD800 cells in nude mice was 3, 7 and 16 d separately, and 3 and 10 d separately in Kunming mice.
Due to its strong tissue penetration, near-infrared fluorescent quantum dots have great prospects in cancer early diagnosis, visual observation and individual treatment.
研究近红外荧光量子点对颊癌的体内可视化成像。
用与细胞穿透肽相连的QD800通过内吞作用标记U14细胞（U14/QD800）。分别将不同数量的U14/QD800注射到裸鼠和昆明小鼠的颊黏膜下，并在不同时间进行成像，以检测U14/QD800的体内敏感性和动态成像。
体内成像系统在裸鼠颊部可检测到的U14/QD800细胞的最小数量为1×10⁴，在昆明小鼠中为1×10⁵。1×10⁴、1×10⁵和1×10⁶个U14/QD800细胞在裸鼠体内的可视化成像时间分别为3、7和16天，在昆明小鼠中分别为3和10天。
近红外荧光量子点因其强大的组织穿透能力，在癌症早期诊断、可视化观察及个体化治疗方面具有广阔前景。
