Universidad de La Salle, Bogotá, Colombia.
Biol Res. 2010;43(2):197-203. Epub 2010 Sep 24.
The objective of this work was to establish, under experimental laboratory conditions, a colony of Lucilia sericata, Bogotá-Colombia strain, to build life tables and evaluate two artificial diets. This blowfly is frequently used in postmortem interval studies and in injury treatment. The parental adult insects collected in Bogotá were maintained in cages at 22°C±1 average temperature, 60%±5 relative humidity and 12 h photoperiodicity. The blowflies were fed on two artificial diets that were evaluated over seven continuous generations. Reproductive and population parameters were assessed. The life cycle of the species was expressed in the number of days of the different stages: egg = 0.8±0.1, larvae I = 1.1±0.02, larvae II = 1.94±0.16, larvae III = 3.5±0.54, pupae = 6.55±0.47, male adult = 28.7±0.83 and female adult = 33.5±1.0. Total survival from egg stage to adult stage was 91.2% for diet 1, while for diet 2 this parameter was 40.5%. The lifetime reproductive output was 184.51±11.2 eggs per female. The population parameters, as well as the reproductive output of the blowflies that were assessed, showed relatively high values, giving evidence of the continuous increase of the strain over the different generations and making possible its maintenance as a stable colony that has lasted for more than two years.
本研究旨在实验室内建立哥伦比亚波哥大品系丝光绿蝇(Lucilia sericata)种群,构建生命表并评估两种人工饲料。这种蝇类常用于死后间隔时间研究和创伤治疗。收集自波哥大的亲代成虫在温度为 22°C±1、相对湿度为 60%±5、光照周期为 12 h 的笼子中饲养。两种人工饲料连续评估了 7 个世代,用于饲养蝇类。评估了繁殖和种群参数。该物种的生命周期用不同阶段的天数表示:卵=0.8±0.1,幼虫 I = 1.1±0.02,幼虫 II = 1.94±0.16,幼虫 III = 3.5±0.54,蛹=6.55±0.47,雄性成虫=28.7±0.83,雌性成虫=33.5±1.0。从卵期到成虫期的总存活率为 184.51±11.2 个卵/雌蝇。种群参数以及评估的蝇类的生殖输出均表现出较高的值,这表明该品系在不同世代中持续增长,使其能够作为一个稳定的种群持续存在,已经持续了两年多。