Bourel B, Hédouin V, Martin-Bouyer L, Bécart A, Tournel G, Deveaux M, Gosset D
Institut de Médecine Légale et de Médecine Sociale, Lille, France.
J Forensic Sci. 1999 Mar;44(2):354-8.
This study concerns the effects of morphine in tissues on the rate of development of Lucilia sericata (Diptera: Calliphoridae) using those tissues as a food source. Lucilia sericata is a species of fly commonly found on human corpses in Europe during the early stages of decomposition and thus of forensic interest. Three rabbits were administered 12.5, 25.0 and 50.0 mg/h of morphine chlorhydrate via ear perfusion over a period of 3 h. These dosages and duration of perfusion were calculated to give tissue concentrations of morphine similar to those encountered in fatal human overdoses. A fourth rabbit was used as a control. Following administration of the drug, rabbits were sacrificed and 400 eggs of Lucilia sericata, all of the same age, were placed in the eyes, nostrils and mouth of each rabbit. Developing larvae were sampled daily to determine growth rate and weight. Puparia and emerging adult flies were also sampled. Data were analyzed using analysis of variance (ANOVA) and Student's T-test. Results of this study show that an underestimation of the postmortem interval of 24 h is possible if the presence of morphine in tissues is not considered. This study demonstrates again the necessity of considering the possible effects of drugs in tissues on insect growth rates when estimating the postmortem interval using entomological techniques.
本研究关注吗啡在组织中的含量对丝光绿蝇（双翅目：丽蝇科）发育速率的影响，该研究将含有吗啡的组织作为丝光绿蝇的食物来源。丝光绿蝇是一种常见于欧洲人类尸体分解早期阶段的苍蝇，因此具有法医学研究价值。三只兔子通过耳灌注在3小时内分别给予12.5、25.0和50.0毫克/小时的盐酸吗啡。计算这些剂量和灌注持续时间，以使组织中的吗啡浓度与人类致命过量用药时的浓度相似。第四只兔子用作对照。给药后，处死兔子，并将400枚相同年龄的丝光绿蝇卵分别置于每只兔子的眼睛、鼻孔和口中。每天对发育中的幼虫进行采样，以确定其生长速率和体重。也对蛹和羽化出的成虫进行采样。使用方差分析（ANOVA）和学生t检验对数据进行分析。本研究结果表明，如果不考虑组织中吗啡的存在，可能会低估24小时的死后间隔时间。本研究再次证明，在使用昆虫学技术估计死后间隔时间时，有必要考虑组织中药物对昆虫生长速率的可能影响。
