Alliance for Research on North Africa, University of Tsukuba, Tsukuba, Japan.
Planta Med. 2011 Mar;77(5):434-40. doi: 10.1055/s-0030-1250402. Epub 2010 Oct 28.
The effect of hot water extracts of LYCIUM CHINENSE fruits (LCF) on the β-hexosaminidase (β-hexo) release by IgE sensitized BSA stimulated rat basophilic leukemia (RBL-2H3) cells was investigated. The ethylacetate (EtOAc) layer of the extract has shown an inhibitory effect on β-hexo release from RBL-2H3 cells at the antigen antibody binding stage. The water (H₂O) fraction (EFW) of the chloroform (CHCl₃) extract from the EtOAc layer also inhibited β-hexo release at the same stage in a dose-dependent manner. With column chromatography preparation, proton and carbon nuclear magnetic resonance (¹H and ¹³C NMR) spectra, electron ionization mass spectrometer (EI-MS) spectra, and high-performance liquid chromatography (HPLC) analysis, the active component was determined to be 5-(hydroxymethyl)furfural (5-HMF). Thus, the 5-HMF showed an inhibitory effect on β-hexo release at the antigen-antibody binding stage and the antibody-receptor binding stage. Furthermore, 5-HMF suppressed [Ca²+] I influx in the IgE-sensitized BSA-stimulated RBL-2H3 cells. Our results show that 5-HMF may be useful for the treatment or prevention of type I allergic diseases.
研究了枸杞果实（LCF）热水提取物对 IgE 致敏 BSA 刺激的大鼠嗜碱性白血病（RBL-2H3）细胞β-己糖胺酶（β-hexo）释放的影响。提取物的乙酸乙酯（EtOAc）层在抗原抗体结合阶段显示出对 RBL-2H3 细胞中β-hexo 释放的抑制作用。氯仿（CHCl₃）提取物的水（H₂O）部分（EFW）也以剂量依赖的方式在同一阶段抑制β-hexo 释放。通过柱色谱制备、质子和碳核磁共振（¹H 和 ¹³C NMR）谱、电子电离质谱（EI-MS）谱和高效液相色谱（HPLC）分析，确定活性成分为 5-（羟甲基）糠醛（5-HMF）。因此，5-HMF 在抗原-抗体结合阶段和抗体-受体结合阶段均显示出抑制β-hexo 释放的作用。此外，5-HMF 抑制了 IgE 致敏 BSA 刺激的 RBL-2H3 细胞中[Ca²+]I 的内流。我们的结果表明，5-HMF 可能对治疗或预防 I 型过敏性疾病有用。
