Pharmacology Division, Indian Institute of Integrative Medicine C.S.I.R., Jammu, India.
Immunol Invest. 2013;42(6):470-80. doi: 10.3109/08820139.2013.789909. Epub 2013 Jun 19.
The present study was designed to investigate the anti-allergic effects of ethanolic extract of Alternanthera sessilis (AS-1) in rat basophilic leukemia (RBL-2H3) cells. It significantly reduced the β-hexosaminidase release from anti-DNP-IgE sensitized RBL-2H3 cells. AS-1also inhibited the IgE antibody-induced increase in Interleukin-6 (IL-6), TNF-α, IL-13 and IL-4 production in these cells. The inhibitory effect of AS-1 on these cytokine was found to be nuclear factor-KB (NF-kB) dependent, as it attenuated the degradation of IKBa and nuclear translocation of NFkB. In addition, AS-1 significantly attenuated the DNP HAS-induced intracellular Ca(2+) release from these cells, which makes us speculate strongly that the decreased intracellular Ca(2+) is involved in the inhibitory effect of AS-1 on β-hexoaminidase release. Taken together, anti-allergic effects of AS-1 suggest possible therapeutic application of this extract in allergic diseases.
本研究旨在探讨节节草（AS-1）乙醇提取物的抗过敏作用及其在大鼠嗜碱性白血病（RBL-2H3）细胞中的作用机制。结果表明，AS-1 可显著减少抗 DNP-IgE 致敏的 RBL-2H3 细胞β-己糖胺酶的释放。AS-1 还可抑制 IgE 抗体诱导的细胞因子白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、IL-13 和 IL-4 的产生。AS-1 对这些细胞因子的抑制作用依赖于核因子-KB（NF-kB），因为它可减弱 IKBa 的降解和 NFkB 的核易位。此外，AS-1 可显著减弱 DNP HAS 诱导的这些细胞内 Ca(2+)的释放，这使我们强烈推测细胞内 Ca(2+)的减少参与了 AS-1 对β-己糖胺酶释放的抑制作用。综上所述，AS-1 的抗过敏作用提示该提取物可能具有治疗过敏疾病的应用价值。
