Natural Products and Medicinal Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Molecules. 2023 Jun 16;28(12):4815. doi: 10.3390/molecules28124815.
Falc. (Synonym: Saussurea costus (Falc.) Lipsch.) is a perennial herb of the family Asteraceae. The dried rhizome is an essential herb in the traditional systems of medicine in India, China and Tibet. The important pharmacological activities reported for are anticancer, hepatoprotective, antiulcer, antimicrobial, antiparasitic, antioxidant, anti-inflammatory and anti-fatigue activities. The objective of this study was the isolation and quantification of four marker compounds in the crude extract and different fractions of and the evaluation of the anticancer activity of the crude extract and its different fractions. The four marker compounds isolated from include dehydrocostus lactone, costunolide, syringin and 5-hydroxymethyl-2-furaldehyde. These four compounds were used as standard compounds for quantification. The chromatographic data showed good resolution and excellent linearity (r ˃ 0.993). The validation parameters, such as inter- and intraday precision (RSD < 1.96%) and analyte recovery (97.52-110.20%; RSD < 2.00%),revealed the high sensitivity and reliability of the developed HPLC method. The compounds dehydrocostus lactone and costunolide were concentrated in the hexane fraction (222.08 and 65.07 µg/mg, respectively) and chloroform fraction (99.02 and 30.21 µg/mg, respectively), while the -butanol fraction is a rich source of syringin (37.91 µg/mg) and 5-hydroxymethyl-2-furaldehyde (7.94 µg/mg). Further, the SRB assay was performed for the evaluation of anticancer activity using lung, colon, breast and prostate cancer cell lines. The hexane and chloroform fractions show excellent IC values of 3.37 ± 0.14 and 7.527 ± 0.18 µg/mL, respectively, against the prostate cancer cell line (PC-3).
雪上一枝蒿（同义词：川西小黄菊（Falc.）Lipsch.）是菊科的一种多年生草本植物。其干燥根茎是印度、中国和西藏传统医学体系中的重要草药。据报道，雪上一枝蒿具有抗癌、保肝、抗溃疡、抗菌、抗寄生虫、抗氧化、抗炎和抗疲劳等重要的药理活性。本研究的目的是从雪上一枝蒿的粗提取物及其不同馏分中分离和定量四种标记化合物，并评估粗提取物及其不同馏分的抗癌活性。从雪上一枝蒿中分离得到的四种标记化合物包括去氢木香内酯、木香烃内酯、丁香苷和 5-羟甲基-2-糠醛。这四种化合物被用作定量的标准化合物。色谱数据显示出良好的分辨率和极好的线性（r ˃ 0.993）。日内和日间精密度（RSD ＜ 1.96%）和分析物回收率（97.52-110.20%；RSD ＜ 2.00%）等验证参数表明，所建立的 HPLC 方法具有较高的灵敏度和可靠性。去氢木香内酯和木香烃内酯在正己烷馏分（分别为 222.08 和 65.07 µg/mg）和氯仿馏分（分别为 99.02 和 30.21 µg/mg）中浓缩，而 -丁醇馏分则是丁香苷（37.91 µg/mg）和 5-羟甲基-2-糠醛（7.94 µg/mg）的丰富来源。此外，还采用 SRB 法评估了肺癌、结肠癌、乳腺癌和前列腺癌细胞系的抗癌活性。正己烷和氯仿馏分对前列腺癌细胞系（PC-3）的 IC 值分别为 3.37 ± 0.14 和 7.527 ± 0.18 µg/mL，显示出优异的活性。
