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Electron transfer dissociation coupled to an Orbitrap analyzer may promise a straightforward and accurate sequencing of disulfide-bridged cyclic peptides: a case study.与轨道阱分析仪联用的电子转移解离技术可能为二硫键桥连环肽的直接且准确测序带来希望：一项案例研究。
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2
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Strategy to improve the quantitative LC-MS analysis of molecular ions resistant to gas-phase collision induced dissociation: application to disulfide-rich cyclic peptides.改善对气相碰撞诱导解离有抗性的分子离子的定量液相色谱-质谱分析的策略：应用于富含二硫键的环肽
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Role of disulfide bonds in folding and activity of leiurotoxin I: just two disulfides suffice.二硫键在类蝎毒素I折叠及活性中的作用：仅两个二硫键就足够了。
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Combined use of ion mobility and collision-induced dissociation to investigate the opening of disulfide bridges by electron-transfer dissociation in peptides bearing two disulfide bonds.联合使用离子淌度和碰撞诱导解离研究带有两个二硫键的肽中电子转移解离打开二硫键。
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Characterization of Disulfide Linkages in Proteins by 193 nm Ultraviolet Photodissociation (UVPD) Mass Spectrometry.利用 193nm 紫外光解（UVPD）质谱法对蛋白质中二硫键的结构分析。
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Reproducible ion-current-based approach for 24-plex comparison of the tissue proteomes of hibernating versus normal myocardium in swine models.基于可重现离子电流的方法用于猪模型中冬眠心肌与正常心肌组织蛋白质组的24重比较。
J Proteome Res. 2014 May 2;13(5):2571-84. doi: 10.1021/pr5000472. Epub 2014 Apr 4.
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Collision-induced dissociation fragmentation inside disulfide C-terminal loops of natural non-tryptic peptides.天然非胰蛋白酶肽中二硫键 C 末端环内的碰撞诱导解离碎裂。
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Nano-scale liquid chromatography/mass spectrometry and on-the-fly orthogonal array optimization for quantification of therapeutic monoclonal antibodies and the application in preclinical analysis.纳升级液相色谱/质谱联用技术和在线正交阵列优化用于治疗性单克隆抗体的定量分析及其在临床前分析中的应用。
J Chromatogr A. 2012 Aug 17;1251:63-73. doi: 10.1016/j.chroma.2012.06.007. Epub 2012 Jun 21.

本文引用的文献

1
Analytical model for rates of electron attachment and intramolecular electron transfer in electron transfer dissociation mass spectrometry.电子转移解离质谱中电子俘获和分子内电子转移速率的分析模型。
J Am Chem Soc. 2010 May 26;132(20):7074-85. doi: 10.1021/ja100240f.
2
Carboxyl-catalyzed prototropic rearrangements in histidine peptide radicals upon electron transfer: effects of peptide sequence and conformation.羧基催化的组氨酸肽自由基在电子转移中的质子重排：肽序列和构象的影响。
J Am Chem Soc. 2009 Nov 18;131(45):16472-87. doi: 10.1021/ja9050229.
3
Dissociation of disulfide-intact somatostatin ions: the roles of ion type and dissociation method.二硫键完整的生长抑素离子的解离：离子类型和解离方法的作用
Rapid Commun Mass Spectrom. 2009 Sep;23(17):2647-55. doi: 10.1002/rcm.4172.
4
Dereplication and de novo sequencing of nonribosomal peptides.非核糖体肽的去重复和从头测序
Nat Methods. 2009 Aug;6(8):596-9. doi: 10.1038/nmeth.1350. Epub 2009 Jul 13.
5
Interpretation of tandem mass spectra obtained from cyclic nonribosomal peptides.从环状非核糖体肽获得的串联质谱的解读。
Anal Chem. 2009 Jun 1;81(11):4200-9. doi: 10.1021/ac900114t.
6
A straightforward and highly efficient precipitation/on-pellet digestion procedure coupled with a long gradient nano-LC separation and Orbitrap mass spectrometry for label-free expression profiling of the swine heart mitochondrial proteome.一种简单高效的沉淀/颗粒上消化程序，结合长梯度纳米液相色谱分离和轨道阱质谱，用于猪心脏线粒体蛋白质组的无标记表达谱分析。
J Proteome Res. 2009 Jun;8(6):2838-50. doi: 10.1021/pr900001t.
7
Determination of eptifibatide concentration in human plasma utilizing the liquid chromatography-tandem mass spectrometry method.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Feb 15;877(5-6):527-32. doi: 10.1016/j.jchromb.2008.12.060. Epub 2009 Jan 6.
8
Mass spectrometric determination of disulfide linkages in recombinant therapeutic proteins using online LC-MS with electron-transfer dissociation.使用在线液相色谱-质谱联用仪结合电子转移解离技术对重组治疗性蛋白质中的二硫键进行质谱测定。
Anal Chem. 2009 Jan 1;81(1):112-22. doi: 10.1021/ac801560k.
9
Accurate localization and relative quantification of arginine methylation using nanoflow liquid chromatography coupled to electron transfer dissociation and orbitrap mass spectrometry.使用纳流液相色谱与电子转移解离和轨道阱质谱联用技术对精氨酸甲基化进行准确的定位和相对定量。
J Am Soc Mass Spectrom. 2009 Mar;20(3):507-19. doi: 10.1016/j.jasms.2008.11.008. Epub 2008 Nov 21.
10
Sequence-scrambling fragmentation pathways of protonated peptides.质子化肽段的序列扰乱碎片化途径。
J Am Chem Soc. 2008 Dec 31;130(52):17774-89. doi: 10.1021/ja805074d.

Electron transfer dissociation coupled to an Orbitrap analyzer may promise a straightforward and accurate sequencing of disulfide-bridged cyclic peptides: a case study.

作者信息

Duan Xiaotao, Engler Frank A, Qu Jun

出版信息

J Mass Spectrom. 2010 Dec;45(12):1477-82. doi: 10.1002/jms.1857.

DOI:10.1002/jms.1857
PMID:21031361
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3012742/
Abstract
摘要