REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
J Mol Recognit. 2010 Nov-Dec;23(6):536-42. doi: 10.1002/jmr.1066.
Iron oxide magnetic nanoparticles (MNPs) were synthesized by the chemical co-precipitation method and coated with gum arabic (GA) by physical adsorption and covalent attachment. Cultures of mammalian cell lines (HEK293, CHO and TE671) were grown in the presence of uncoated and GA-coated MNPs. Cellular growth was followed by optical microscopy in order to assess the proportion of cells with particles, alterations in cellular density and the presence of debris. The in vitro assays demonstrated that cells from different origins are affected differently by the presence of the nanoparticles. Also, the methods followed for GA coating of MNPs endow distinct surface characteristics that probably underlie the observed differences when in contact with the cells. In general, the nanoparticles to which the GA was adsorbed had a smaller ability to attach to the cells' surface and to compromise the viability of the cultures.
采用化学共沉淀法合成了氧化铁磁性纳米粒子（MNPs），并通过物理吸附和共价附着用阿拉伯树胶（GA）对其进行了包覆。在存在未包覆和 GA 包覆的 MNPs 的情况下，培养哺乳动物细胞系（HEK293、CHO 和 TE671）。通过光学显微镜观察细胞生长情况，以评估带有颗粒的细胞比例、细胞密度变化和碎片存在情况。体外试验表明，不同来源的细胞受到纳米颗粒的影响不同。此外，用于 MNPs 的 GA 包覆所遵循的方法赋予了不同的表面特性，这可能是与细胞接触时观察到差异的基础。一般来说，被 GA 吸附的纳米粒子对细胞表面的附着能力较弱，对培养物的存活率影响较小。
