Gupta Ajay Kumar, Gupta Mona
Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK.
Biomaterials. 2005 May;26(13):1565-73. doi: 10.1016/j.biomaterials.2004.05.022.
The aim of this study was to modify the surfaces of superparamagnetic iron oxide nanoparticles (SPION) with pullulan in order to reduce the cytotoxicity and enhance the cellular uptake of the nanoparticles. In this study, we have prepared and characterised the pullulan coated superparamagnetic iron oxide nanoparticles (Pn-SPION) of size around 40-45 nm with magnetite inner core and hydrophilic outer shell of pullulan. We have investigated the effect of cellular uptake of uncoated and Pn-SPION on cell adhesion/viability, cytotoxicity, morphology and cytoskeleton organisation of human fibroblasts. Cell cytotoxicity/adhesion studies of SPIONs on human dermal fibroblasts showed that the particles are toxic and their internalisation resulted in disruption of cytoskeleton organisation of cells. On the other hand, Pn-SPIONs were found to be non-toxic and induced changes in cytoskeleton organisation different from that observed with SPION. Transmission electron microscopy results indicated that the SPION and Pn-SPION were internalised into cells via different mechanisms, thereby suggesting that the particle endocytosis behaviour is dependent on the surface characteristics of the nanoparticles.
本研究的目的是用支链淀粉修饰超顺磁性氧化铁纳米颗粒（SPION）的表面，以降低细胞毒性并增强纳米颗粒的细胞摄取。在本研究中，我们制备并表征了支链淀粉包被的超顺磁性氧化铁纳米颗粒（Pn-SPION），其尺寸约为40-45nm，内核为磁铁矿，外壳为亲水性的支链淀粉。我们研究了未包被的和Pn-SPION的细胞摄取对人成纤维细胞的细胞黏附/活力、细胞毒性、形态和细胞骨架组织的影响。SPION对人皮肤成纤维细胞的细胞毒性/黏附研究表明，这些颗粒具有毒性，其内化导致细胞骨架组织的破坏。另一方面，发现Pn-SPION无毒，且诱导的细胞骨架组织变化与SPION观察到的不同。透射电子显微镜结果表明，SPION和Pn-SPION通过不同机制内化到细胞中，从而表明颗粒的内吞行为取决于纳米颗粒的表面特性。
