Department of Environmental Engineering Sciences, University of Florida, Gainesville, FL, USA.
Inhal Toxicol. 2011 Aug;23(9):532-43. doi: 10.3109/08958378.2011.592869.
Nanoparticles are being rapidly assimilated into numerous research fields and consumer products. A concurrent increase in human exposure to such materials is expected. Magnetic nanoparticles (MNPs) possess unique and beneficial features, increasing their functionality and integrative potential. However, MNP toxicity characterization is limited, especially in regards to the human respiratory system. This study aimed to assess the in vitro effects of airborne MNPs on BEAS-2B cells. Uncoated iron oxide was compared with two amorphous silica-coated MNPs, hypothesizing the coatings reduced toxicity and increased particle stability.
BEAS-2B cells were cultured at an air-liquid interface and exposed to airborne MNPs using a fabricated exposure device. Indices of cytotoxicity, inflammatory response, oxidative stress, and iron homeostasis were monitored postexposure via cell viability assays and qRT-PCR. Concentrations of soluble iron-associated with different MNPs were also examined before and after contact with several aqueous organic and inorganic acids.
The silica-coated MNPs had reduced soluble iron concentrations. This result indicates that the silica coating provides a barrier to and prevents the mobilization of soluble iron from the particle to the cell, thereby reducing the risk of oxidative stress or alterations of iron homeostasis. Cells exposed to MagSilica50 and MagSilica50-85® showed little to no indications of cytotoxicity or induction of inflammatory response/oxidative stress at the examined delivery concentrations.
MNPs coated with amorphous silica are protected from acidic erosion. Correspondingly, the particle stability translates into reduced cytotoxicity and cellular influence on human airway epithelial cells.
纳米颗粒正在迅速融入众多研究领域和消费产品。预计人类接触此类材料的人数也将同时增加。磁性纳米颗粒 (MNP) 具有独特且有益的特性，增加了其功能和综合潜力。然而，MNP 的毒性特征描述有限，特别是在涉及人类呼吸系统方面。本研究旨在评估空气中的 MNP 对 BEAS-2B 细胞的体外影响。我们比较了未涂层的氧化铁与两种非晶态二氧化硅涂层的 MNP，假设涂层可以降低毒性并提高颗粒稳定性。
使用制造的暴露装置，在气液界面培养 BEAS-2B 细胞，并使细胞暴露于空气中的 MNP。通过细胞活力测定和 qRT-PCR 监测暴露后细胞毒性、炎症反应、氧化应激和铁稳态的变化。还在与几种水性有机和无机酸接触前后检查了与不同 MNP 相关的可溶性铁的浓度。
二氧化硅涂层的 MNP 具有较低的可溶性铁浓度。这一结果表明，二氧化硅涂层提供了一个屏障，防止了从颗粒到细胞的可溶性铁的迁移，从而降低了氧化应激或铁稳态改变的风险。暴露于 MagSilica50 和 MagSilica50-85® 的细胞在检查的输送浓度下几乎没有表现出细胞毒性或诱导炎症反应/氧化应激的迹象。
用非晶态二氧化硅涂层的 MNP 受到酸性侵蚀的保护。相应地，颗粒稳定性转化为降低的细胞毒性和对人呼吸道上皮细胞的细胞影响。
