Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, Spain.
J Comp Neurol. 2010 Dec 15;518(24):4917-37. doi: 10.1002/cne.22496.
Certain GABAergic interneurons in the cerebral cortex, basket cells, establish multiple connections with cell bodies that typically outline the somata and proximal dendrites of pyramidal cells. During studies into the distribution of the vesicular GABA transporter (VGAT) in the human cerebral cortex, we were struck by the presence of a very dense, pericellular arrangement of multiple VGAT-immunoreactive (-ir) terminals in certain cortical areas. We called these terminals "Complex basket formations" (Cbk-formations) to distinguish them from the simpler and more typical pericellular GABAergic innervations of most cortical neurons. Here we examined the distribution of these VGAT-ir Cbk-formations in various cortical areas, including the somatosensory (area 3b), visual (areas 17 and 18), motor (area 4), associative frontal (dorsolateral areas 9, 10, 45, 46, and orbital areas 11, 12, 13, 14, 47), associative temporal (areas 20, 21, 22, and 38), and limbic cingulate areas (areas 24, 32). Furthermore, we used dual or triple staining techniques to study the chemical nature of the innervated cells. We found that VGAT-ir Cbk-formations were most frequently found in area 4 followed by areas 3b, 13, and 18. In addition, they were mostly observed in layer III, except in area 17, where they were most dense in layer IV. We also found that 70% of the innervated neurons were pyramidal cells, while the remaining 30% were multipolar cells. Most of these multipolar cells expressed the calcium-binding protein parvalbumin and the lectin Vicia villosa agglutinin.
大脑皮层中的某些 GABA 能中间神经元，篮状细胞，与细胞体建立多个连接，这些细胞体通常勾勒出锥体神经元的胞体和近端树突。在研究人类大脑皮层中囊泡 GABA 转运体 (VGAT) 的分布时，我们注意到在某些皮层区域存在一种非常密集的、围绕细胞的多个 VGAT 免疫反应 (-ir) 末端的排列。我们称这些末端为“复杂篮状结构”（Cbk-formation），以将它们与大多数皮质神经元更简单和更典型的围绕细胞的 GABA 能神经支配区分开来。在这里，我们检查了这些 VGAT-ir Cbk-formation 在各种皮层区域的分布，包括躯体感觉（区域 3b）、视觉（区域 17 和 18）、运动（区域 4）、联合额前（背外侧区域 9、10、45、46 和眶部 11、12、13、14、47）、联合颞叶（区域 20、21、22 和 38）和边缘扣带回区域（区域 24、32）。此外，我们使用双重或三重染色技术来研究受神经支配细胞的化学性质。我们发现，VGAT-ir Cbk-formation 最常见于区域 4，其次是区域 3b、13 和 18。此外，它们主要存在于第三层，除了在区域 17 中，它们在第四层最密集。我们还发现，70%的受神经支配的神经元是锥体神经元，而其余 30%是多极细胞。这些多极细胞中的大多数表达钙结合蛋白 parvalbumin 和植物凝集素 Vicia villosa agglutinin。
