Department of Hepatology and Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, PR China.
Phytother Res. 2010 Nov;24(11):1627-30. doi: 10.1002/ptr.3155.
Despite the availability of an effective vaccine, the hepatitis B virus (HBV) infection and its treatment remains one of the foremost public health problems in the world. The present study was performed in order to investigate the anti-HBV activity of lutein in vitro. The antiviral activity of lutein was examined by detecting the levels of HBsAg, HBeAg and extracellular HBV DNA in stable HBV-producing human hepatoblastoma HepG2 2.2.15 cells. It was found that lutein effectively suppressed the secretion of HBsAg from HepG2 2.2.15 cells in a dose-dependent manner, and it also suppressed the amount of extracellular HBV DNA. A luciferase reporter gene assay was used to determine the effects of lutein on the activities of HBV promoters. The results showed that lutein inhibited the activity of HBV full-length promoter (Fp). These data indicate that lutein possesses an anti-HBV activity and exerts its antivirus effects via inhibition of HBV transcription.
尽管已有有效的疫苗，但乙型肝炎病毒 (HBV) 感染及其治疗仍然是世界上最重要的公共卫生问题之一。本研究旨在体外研究叶黄素的抗 HBV 活性。通过检测稳定产生 HBV 的人肝癌 HepG2 2.2.15 细胞中的 HBsAg、HBeAg 和细胞外 HBV DNA 水平来检测叶黄素的抗病毒活性。结果发现，叶黄素能有效抑制 HepG2 2.2.15 细胞中 HBsAg 的分泌，且呈剂量依赖性，还能抑制细胞外 HBV DNA 的含量。采用荧光素酶报告基因检测来确定叶黄素对 HBV 启动子活性的影响。结果表明，叶黄素抑制 HBV 全长启动子 (Fp) 的活性。这些数据表明，叶黄素具有抗 HBV 活性，通过抑制 HBV 转录发挥其抗病毒作用。
