Department of Animal Biotechnology, College of Veterinary Science and A.H., Anand Agricultural University, Anand 388 001, Gujarat, India.
Pol J Microbiol. 2010;59(3):175-8.
Methane emissions from ruminant livestock are considered to be one of the more potent forms of greenhouse gases contributing to global warming. Many strategies to reduce emissions are targeting the methanogens that inhabit the rumen, but such an approach can only be successful if it targets all the major groups of ruminant methanogens. Therefore, basic knowledge of the diversity of these microbes in breeds of buffalo is required. Therefore, the methanogenic community in the rumen of Surti buffaloes was analyzed by PCR amplification, cloning, and sequencing of methyl coenzyme M reductase (mcrA) gene. A total of 76 clones were identified, revealing 14 different sequences (phylotypes). All 14 sequences were similar to methanogens belonging to the order Methanobacteriales. Within Methanobacteriales, 12 clones (6 OTUs) were similar to Methanosphaera stadtmanae and the remaining 8 phylotypes (64 clones) were similar to unclassified Methanobacteriales. Overall, members of the Methanobacteriales dominated the mcrA clone library in the rumen of Surti buffalo. Further studies and effective strategies can be made to inhibit the growth of Methanobacteriales to reduce methane emission from the rumen which would help in preventing global warming.
反刍动物的甲烷排放被认为是导致全球变暖的更强烈的温室气体之一。许多减排策略都针对栖息在瘤胃中的产甲烷菌，但如果这种方法要成功，就必须针对反刍动物产甲烷菌的所有主要群体。因此，需要了解水牛品种中这些微生物的多样性的基础知识。因此，通过聚合酶链反应扩增、克隆和测序甲基辅酶 M 还原酶（mcrA）基因，分析了印度 Surti 水牛瘤胃中的产甲烷群落。共鉴定出 76 个克隆，揭示了 14 种不同的序列（系统发育型）。所有 14 个序列与属于甲烷杆菌目（Methanobacteriales）的产甲烷菌相似。在甲烷杆菌目中，12 个克隆（6 个 OTUs）与 Methanosphaera stadtmanae 相似，其余 8 个系统发育型（64 个克隆）与未分类的甲烷杆菌目相似。总体而言，Methanobacteriales 的成员在 Surti 水牛瘤胃的 mcrA 克隆文库中占主导地位。可以进一步研究并制定有效的策略来抑制甲烷杆菌目（Methanobacteriales）的生长，以减少瘤胃中的甲烷排放，从而有助于防止全球变暖。
