Nutrition Biotechnology Lab, Animal Biotechnology Centre, National Dairy Research Institute, Karnal-132001, Haryana, India.
J Microbiol. 2011 Aug;49(4):558-61. doi: 10.1007/s12275-011-1052-y. Epub 2011 Sep 2.
The aim of the present study was to decipher the diversity of methanogens in rumen of Murrah buffaloes so that effective strategies can be made in order to mitigate methane emission from these methanogens. In the present study diversity of rumen methanogens in Murrah buffaloes (Bubalus bubalis) from North India was evaluated by using mcr-A gene library obtained from the pooled PCR product from four animals and by using MEGA4 software. A total of 104 clones were examined, revealing 26 different mcr-A gene sequences or phylotypes. Of the 26 phylotypes, 16 (64 of 104 clones) were less than 97% similar to any of the cultured strain of methanogens. Seven clone sequences were clustered with Methanomicrobium mobile and three clone sequences were clustered with Methanobrevibacter gottschalkii during the phylogenetic analysis. Uncultured group of methanogens comes out to be the major component of the methanogens community structure in Murrah buffaloes. Methanomicrobium phylotype comes out to be major phylotype among cultured methanogens followed by Methanobrevibacter phylotype. These results help in making effective strategies to check the growth of dominant communities in the rumen of this animal which in turn help in the reduction of methane emission in the environment and ultimately helps us in fighting with the problem of global warming.
本研究旨在揭示印度北方瘤胃液中甲烷菌的多样性，以便制定有效的策略来减轻这些甲烷菌的甲烷排放。本研究采用从 4 只动物的聚合 PCR 产物中获得的 mcr-A 基因文库，应用 MEGA4 软件，评估了印度北方瘤胃液中瘤胃甲烷菌的多样性。共检查了 104 个克隆，揭示了 26 种不同的 mcr-A 基因序列或系统发育型。在 26 种系统发育型中，有 16 种（104 个克隆中的 64 种）与任何已培养的甲烷菌菌株的相似度低于 97%。在系统发育分析中，7 个克隆序列与 Methanomicrobium mobile 聚类，3 个克隆序列与 Methanobrevibacter gottschalkii 聚类。未培养的甲烷菌群体是瘤胃甲烷菌群落结构的主要组成部分。在已培养的甲烷菌中，Methanomicrobium 系统发育型是主要的系统发育型，其次是 Methanobrevibacter 系统发育型。这些结果有助于制定有效的策略来控制该动物瘤胃中优势菌群的生长，从而有助于减少环境中的甲烷排放，最终有助于我们解决全球变暖问题。
