Golz C, Köhler F, Schieder O
Institut für Angewandte Genetik, FU Berlin, FRG.
Plant Mol Biol. 1990 Sep;15(3):475-83. doi: 10.1007/BF00019164.
The successful transfer of a marker gene (hpt gene) from Brassica nigra into B. napus via direct gene transfer was demonstrated. Total DNA was isolated from a hygromycin-resistant callus line, which contained three to five copies of the hpt gene. This line had been produced via direct gene transfer with the hygromycin resistance-conferring plasmid pGL2. The treatment of B. napus protoplasts with genomic DNA of B. nigra (HygR) resulted in relative transformation frequencies of 0.1-0.4%. Similar transformation rates were obtained in direct gene transfer experiments using B. napus protoplasts and plasmid pGL2.
已证实通过直接基因转移成功地将标记基因（hpt基因）从黑芥转移到甘蓝型油菜中。从一个抗潮霉素愈伤组织系中分离出总DNA，该系含有三到五个拷贝的hpt基因。该系是通过用赋予潮霉素抗性的质粒pGL2进行直接基因转移产生的。用黑芥（HygR）的基因组DNA处理甘蓝型油菜原生质体，相对转化频率为0.1 - 0.4%。在使用甘蓝型油菜原生质体和质粒pGL2的直接基因转移实验中也获得了类似的转化率。
