生物聚合物包封活流感病毒作为通用的流感病毒 CD8+ T 细胞疫苗。
Biopolymer encapsulated live influenza virus as a universal CD8+ T cell vaccine against influenza virus.
机构信息
Department of Microbiology and Immunology, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA.
出版信息
Vaccine. 2010 Dec 16;29(2):314-22. doi: 10.1016/j.vaccine.2010.10.036. Epub 2010 Oct 27.
Abstract
Current influenza virus vaccines primarily elicit antibodies and can be rendered ineffective by antigenic drift and shift. Vaccines that elicit CD8+ T cell responses targeting less variable proteins may function as universal vaccines that have broad reactivity against different influenza virus strains. To generate such a universal vaccine, we encapsulated live influenza virus in a biopolymer and delivered it to mice subcutaneously. This vaccine was safe, induced potent CD8+ T cell immunity and protected mice against heterosubtypic lethal challenge. Safety of subcutaneous (SQ) vaccination was tested in Rag-/-γc-/- double knockout mice which we show cannot control intranasal infection. Biopolymer encapsulation of live influenza virus could be used to develop universal CD8+ T cell vaccines against heterosubtypic and pandemic strains.
摘要
当前的流感病毒疫苗主要诱导产生抗体，并且可能因抗原漂移和转变而失效。诱导针对较少变异蛋白的 CD8+T 细胞反应的疫苗可能作为通用疫苗发挥作用，对不同的流感病毒株具有广泛的反应性。为了产生这样的通用疫苗，我们将活流感病毒包裹在生物聚合物中，并通过皮下途径将其递送到小鼠体内。这种疫苗是安全的，能够诱导强烈的 CD8+T 细胞免疫反应，并保护小鼠免受异源亚致死性攻击。我们在 Rag-/-γc-/-双重基因敲除小鼠中测试了皮下（SQ）接种的安全性，这些小鼠不能控制鼻内感染。活流感病毒的生物聚合物包封可用于开发针对异源和大流行株的通用 CD8+T 细胞疫苗。
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