Department of Dental Materials, School of Dentistry, University of Granada, Granada, Spain.
J Dent. 2011 Jan;39(1):80-7. doi: 10.1016/j.jdent.2010.10.013. Epub 2010 Oct 28.
To determine the role of an anti-oxidant agent (ascorbic acid-AA) on resin-dentin bonds resistance to degradation of two adhesives.
Flat dentin surfaces from 48 human molars were bonded as per manufacturer's instructions with: a two-step etch-and-rinse self-priming adhesive (Single Bond-SB) and a two-step self-etch adhesive (Clearfil SE Bond-CSE). Half of the specimens were bonded with the same adhesives, but after the addition of 10% AA into their formulation. Resin composite build-ups were constructed incrementally and sectioned into resin-dentin beams (1.0mm(2)) that were stored under four conditions: (1) water immersion for 24h; (2) water immersion for 1 year; (3) water immersion for 4 years; and (4) chemical challenging (immersion in 10% NaOCl for 5 h). Beams were pulled to failure in tension at 0.5mm/min. Mean microtensile bond strength (MTBS) data were analysed with ANOVA and multiple comparisons tests (P<0.05). Analysis of debonded dentin beams was performed by scanning electron microscopy (SEM).
After 24 h, SB and CSE performed equally, regardless of AA incorporation. Inclusion of AA on CSE formulation decreased MTBS following one-year water storage, but maintained SB bond strength values even after four years of water storage. NaOCl challenge diminished MTBS for both adhesives, but when AA was added to SB, this reduction was significantly lower.
The inclusion of AA on adhesive's formulation exerts a protective role on resin-dentin bonds resistance to degradation when SB is used. Bonding durability of CSE may be compromised by the addition of sodium ascorbate.
确定抗氧化剂（抗坏血酸-AA）对两种胶粘剂的树脂-牙本质结合体抗降解能力的作用。
48 个人类磨牙的平坦牙本质表面按照制造商的说明进行粘接：两步酸蚀-冲洗自酸蚀底漆（Single Bond-SB）和两步自酸蚀胶粘剂（Clearfil SE Bond-CSE）。一半的样本用相同的胶粘剂进行粘接，但在其配方中添加了 10% AA。树脂复合材料以增量方式构建，并切成树脂-牙本质梁（1.0mm(2))，然后在以下四种条件下储存：（1）水浸泡 24 小时；（2）水浸泡 1 年；（3）水浸泡 4 年；和（4）化学挑战（在 10%次氯酸钠中浸泡 5 小时）。梁以 0.5mm/min 的速度在张力下断裂。使用方差分析和多重比较检验（P<0.05）分析平均微拉伸结合强度（MTBS）数据。通过扫描电子显微镜（SEM）分析脱粘的牙本质梁。
在 24 小时后，无论是否添加 AA，SB 和 CSE 的性能都相当。CSE 配方中添加 AA 会降低一年水储存后的 MTBS，但即使在四年水储存后仍能保持 SB 结合强度值。次氯酸钠挑战降低了两种胶粘剂的 MTBS，但当 SB 中添加 AA 时，这种降低幅度显著降低。
当使用 SB 时，胶粘剂配方中添加 AA 对树脂-牙本质结合体的抗降解能力发挥保护作用。添加抗坏血酸钠可能会损害 CSE 的粘结耐久性。
