Institute of Biomedical Engineering, National Cheng Kung University, No. 1, University Road, Tainan 70101, Taiwan.
Biosens Bioelectron. 2011 Jan 15;26(5):2334-40. doi: 10.1016/j.bios.2010.10.006. Epub 2010 Oct 14.
Silane-based methods have become the standards for the conjugation of biomolecules, especially for the preparation of one-dimensional nanomaterial biosensors. However, the specific binding of those target molecules might raise problems with regard to the sensing and non-sensing regions, which may contaminate the sensing devices and decrease their sensitivity. This paper attempts to explore the encapsulation of biomolecules on a one-dimensional nanomaterial field effect transistor (FET) biosensor using polypyrrole propylic acid (PPa). Specifically, the encapsulation of biomolecules via the electropolymerization of pyrrole propylic acid (Pa), a self-made low-conductivity polymer, on TiO(2)-nanowire (NW)-based FETs is presented. The energy dispersive spectrum (EDS) was obtained and electrical analysis was conducted to investigate PPa entrapping anti-rabbit IgG (PPa/1°Ab) on a composite film. The specificity, selectivity and sensitivity of the sensor were analyzed in order to determine the immunoreaction of PPa/1°Ab immobilized NW biosensors. Our results show that PPa/1°Ab achieved high specificity immobilization on NWs under the EDS analysis. Furthermore, the TiO(2)-NW FET immunosensor developed in this work successfully achieved specificity, selectivity and sensitivity detection for the target protein rabbit IgG at the nano-gram level. The combination of PPa material and the electropolymerization method may provide an alternative method to immobilize biomolecules on a specific surface, such as NWs.
硅烷法已成为生物分子偶联的标准方法，尤其适用于一维纳米材料生物传感器的制备。然而，那些目标分子的特异性结合可能会在传感和非传感区域引发问题，这可能会污染传感设备并降低其灵敏度。本文试图探索使用聚吡咯丙酸（PPa）在一维纳米材料场效应晶体管（FET）生物传感器上封装生物分子。具体来说，通过将自制低电导率聚合物吡咯丙酸（Pa）在基于 TiO2 纳米线（NW）的 FET 上进行电聚合，实现了生物分子的封装。获得了能谱（EDS）并进行了电分析，以研究复合薄膜上的抗兔 IgG（PPa/1°Ab）包埋情况。为了确定 PPa/1°Ab 固定化 NW 生物传感器的免疫反应，分析了传感器的特异性、选择性和灵敏度。我们的结果表明，在 EDS 分析下，PPa/1°Ab 可以在 NW 上实现高特异性固定化。此外，本工作中开发的 TiO2-NW FET 免疫传感器成功地实现了纳米级目标蛋白兔 IgG 的特异性、选择性和灵敏度检测。PPa 材料与电聚合方法的结合可能为在特定表面（如 NWs）上固定生物分子提供一种替代方法。
