INRA, UR407, Plant Pathology Unit, Domaine St Maurice, F-84140 Montfavet, France.
Fungal Biol. 2010 Nov-Dec;114(11-12):949-54. doi: 10.1016/j.funbio.2010.09.004. Epub 2010 Sep 15.
The stability of microsatellite markers was investigated in the spore-producing fungus Botrytis cinerea exposed to four growth conditions. This knowledge is essential in order to differentiate mutations from genetic exchanges or recombination in population genetics studies. It is also important when using strains from collections that need to be regularly propagated on medium. Successive spore generations of four isolates of the fungus were realised in plates on different agar media: a nutrient-rich medium, a nutrient-poor medium, a medium supplemented with the antibiotic pyrrolnitrin and a medium supplemented with the fungicide iprodione. The stability of nine microsatellite markers was studied by comparing the molecular pattern obtained between the wild type parent strains and the final generations obtained. The results showed that, despite the phenotypic changes observed in some generations, no changes were observed in the allele size at nine microsatellite loci whatever the selective pressure endured by the fungus. This is the first study that reveals long-term stability of microsatellite markers of a spore-producing fungus exposed to different stresses.
本研究调查了在四种生长条件下产孢真菌 Botrytis cinerea 中微卫星标记的稳定性。在群体遗传学研究中，为了区分突变与遗传交换或重组，这一知识是必不可少的。在使用需要定期在培养基上繁殖的培养物时，这一点也很重要。在不同琼脂培养基的平板上实现了四种真菌分离株的连续孢子世代：一种营养丰富的培养基、一种营养贫瘠的培养基、一种添加抗生素吡咯菌素的培养基和一种添加杀菌剂异菌脲的培养基。通过比较野生型亲本菌株和最终获得的世代之间获得的分子图谱，研究了 9 个微卫星标记的稳定性。结果表明，尽管在某些世代中观察到表型变化，但无论真菌承受何种选择压力，9 个微卫星位点的等位基因大小均未观察到变化。这是第一项揭示产孢真菌在不同胁迫下微卫星标记长期稳定性的研究。
