Phytopathology. 2014 Aug;104(8):859-64. doi: 10.1094/PHYTO-10-13-0271-R.
Although Botrytis cinerea is known for its ability to produce high amounts of spores on diseased plants, enabling it to complete rapidly numerous developmental cycles in favorable environments, population genetics studies of this fungus indicate enormous diversity and limited clonal spread. Here, we report an exception to this situation in the settings of commercial tomato greenhouses. The genotypic characterization of 712 isolates collected from the air and from diseased plants, following the development of gray mold epidemics in four greenhouses in southern France, revealed the presence of a few predominant genotypes in a background of highly diverse populations. The comparison of genotypic profiles for isolates collected in the air or on the plants was compatible with the hypothesis of an entry in the greenhouse of substantial amounts of inoculum from the outside environment but it also highlighted the importance of secondary inoculum produced within the crop. The overall results of this work suggest that sporulation could be an important target for disease management strategies in the greenhouse.
虽然灰葡萄孢（Botrytis cinerea）以在病株上产生大量孢子的能力而闻名，使其能够在有利环境中迅速完成多次发育周期，但对该真菌的种群遗传学研究表明，其具有巨大的多样性和有限的无性系传播。在这里，我们报告了在商业番茄温室环境中的一个例外情况。在法国南部的四个温室中发生灰霉病流行后，从空气和病株中收集的 712 个分离物的基因型特征表明，在高度多样化的种群背景下存在少数主要基因型。对在空气中或植物上收集的分离物的基因型图谱进行比较，与从外部环境中大量接种体进入温室的假设一致，但也强调了作物内产生的次要接种体的重要性。这项工作的总体结果表明，孢子形成可能是温室病害管理策略的一个重要目标。
