Langness U, Clausnitzer H, Verspohl M, Grasedyck K
Med Klin. 1978 Aug 25;73(34):1179-82.
100 patients were laparoscopied, liver tissue specimens taken from atypically altered areas. Prolyl hydroxylase was determined in the specimen, in parallel tissue was examined by light microscope. 8 groups of patients could be differentiated: Patients 1. with active, 2, with inactive cirrhosis, 3. with fatty infiltrations, 4. with fatty infiltration and mesenchymal reaction, 5. with aggressive, 6. with persistent, 7. with reactive hepatitis, 8. patients without histological changes. In the case of connective tissue increase in the liver prolyl hydroxylase activities were statistically significant above normal. In addition, there was a statistically significant difference between the enzyme activities of each group. A correlation could be found between prolyl hydroxylase activity and morphologically estimated connective tissue formation, but not the serum enzyme activities usually determined in liver diseases. Therefore, could be concluded that prolyl hydroxylase activity is an index of actual collagen biosynthesis in chronic liver diseases.
对100例患者进行了腹腔镜检查，从非典型改变区域获取肝组织标本。测定标本中的脯氨酰羟化酶，同时用光学显微镜检查平行组织。可将患者分为8组：1. 活动性肝硬化患者；2. 非活动性肝硬化患者；3. 脂肪浸润患者；4. 脂肪浸润伴间充质反应患者；5. 侵袭性肝炎患者；6. 持续性肝炎患者；7. 反应性肝炎患者；8. 无组织学改变的患者。在肝脏结缔组织增加的情况下，脯氨酰羟化酶活性在统计学上显著高于正常水平。此外，各组的酶活性之间存在统计学上的显著差异。脯氨酰羟化酶活性与形态学估计的结缔组织形成之间存在相关性，但与通常在肝病中测定的血清酶活性无关。因此，可以得出结论，脯氨酰羟化酶活性是慢性肝病中实际胶原生物合成的一个指标。
