Plant Stress Response Group, Department of Biochemistry & Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
Comput Biol Chem. 2010 Dec;34(5-6):268-83. doi: 10.1016/j.compbiolchem.2010.09.003. Epub 2010 Oct 30.
The regulation of gene expression involves a multifarious regulatory system. Each gene contains a unique combination of cis-acting regulatory sequence elements in the 5' regulatory region that determines its temporal and spatial expression. Cis-acting regulatory elements are essential transcriptional gene regulatory units; they control many biological processes and stress responses. Thus a full understanding of the transcriptional gene regulation system will depend on successful functional analyses of cis-acting elements. Cis-acting regulatory elements present within the 5' regulatory region of the sucrose transporter gene families in rice (Oryza sativa Japonica cultivar-group) and Arabidopsis thaliana, were identified using a bioinformatics approach. The possible cis-acting regulatory elements were predicted by scanning 1.5kbp of 5' regulatory regions of the sucrose transporter genes translational start sites, using Plant CARE, PLACE and Genomatix Matinspector professional databases. Several cis-acting regulatory elements that are associated with plant development, plant hormonal regulation and stress response were identified, and were present in varying frequencies within the 1.5kbp of 5' regulatory region, among which are; A-box, RY, CAT, Pyrimidine-box, Sucrose-box, ABRE, ARF, ERE, GARE, Me-JA, ARE, DRE, GA-motif, GATA, GT-1, MYC, MYB, W-box, and I-box. This result reveals the probable cis-acting regulatory elements that possibly are involved in the expression and regulation of sucrose transporter gene families in rice and Arabidopsis thaliana during cellular development or environmental stress conditions.
基因表达的调控涉及一个多样化的调控系统。每个基因在其 5'调控区都包含一个独特的顺式作用调控序列元件组合，决定其时空表达。顺式作用调控元件是转录基因调控的基本单元；它们控制着许多生物过程和应激反应。因此，对转录基因调控系统的全面理解将取决于对顺式作用元件的成功功能分析。
采用生物信息学方法鉴定了水稻（粳稻品种组）和拟南芥蔗糖转运基因家族 5'调控区中的顺式作用调控元件。通过扫描蔗糖转运基因翻译起始位点 5'调控区 1.5kbp，使用 Plant CARE、PLACE 和 Genomatix Matinspector 专业数据库，预测了可能的顺式作用调控元件。鉴定出了与植物发育、植物激素调节和应激反应相关的几个顺式作用调控元件，并在 1.5kbp 的 5'调控区内以不同的频率存在，其中包括：A-box、RY、CAT、嘧啶盒、蔗糖盒、ABRE、ARF、ERE、GARE、Me-JA、ARE、DRE、GA-motif、GATA、GT-1、MYC、MYB、W-box 和 I-box。
该结果揭示了可能参与水稻和拟南芥蔗糖转运基因家族在细胞发育或环境胁迫条件下表达和调控的顺式作用调控元件。
