Nunes Sávio Luiz Pereira, Soares Julianna Matos da Silva, Rocha Anelita de Jesus, Nascimento Fernanda Dos Santos, Ramos Andresa Priscila de Souza, Soares Taliane Leila, Santos Rogério Merces Ferreira, Amorim Vanusia Batista de Oliveira, Amorim Edson Perito, Ferreira Claudia Fortes
Department of Plant Genetic Resources, Federal University of the Reconcavo of Bahia, Cruz das Almas 44380-000, Bahia, Brazil.
Department of Biological Sciences, Feira de Santana State University, Feira de Santana 44036-900, Bahia, Brazil.
Curr Issues Mol Biol. 2024 Dec 11;46(12):13991-14009. doi: 10.3390/cimb46120837.
This work aimed to evaluate the relative gene expression of the candidate genes , , , , and involved in the defense response to Black Sigatoka in banana cultivars Calcutta-4, Krasan Saichon, Grand Nain, and Akondro Mainty, by a quantitative real-time PCR. Biotic stress was imposed on 6-month-old plants during five sampling intervals under greenhouse conditions. The and genes were upregulated for the Calcutta-4- and Krasan Saichon-resistant cultivars, and were validated in this study. For Grande Naine, a susceptible cultivar, there was an early downregulation of the , , and genes and a late upregulation of the gene. There was no significant expression of any of the genes for the susceptible cultivar Akondro Mainty. Computational biology tools such as ORFFinder and PlantCARE revealed that the utp gene has more introns and exons and that, in general, cis-elements involved in the response to biotic stress, such as as-1, w-box, and STRE, were detected in the promoter region of the genes studied. Data from this work also support the phenotyping studies of banana cultivars affected by Black Sigatoka in the field. Once validated in promising new hybrids, these genes may be used in marker-assisted selection (MAS) and/or gene-editing techniques.
本研究旨在通过定量实时聚合酶链反应（qRT-PCR）评估香蕉品种加尔各答4号、克拉桑赛琼、大麦克和阿孔德罗曼蒂中参与抵御香蕉叶斑病防御反应的候选基因、、、、和的相对基因表达。在温室条件下，对6个月大的植株在五个采样间隔期施加生物胁迫。和基因在抗香蕉叶斑病的加尔各答4号和克拉桑赛琼品种中上调，并在本研究中得到验证。对于感病品种大麦克，、和基因早期下调，基因后期上调。对于感病品种阿孔德罗曼蒂，任何基因均无显著表达。诸如开放阅读框查找器（ORFFinder）和植物顺式作用元件数据库（PlantCARE）等计算生物学工具显示，utp基因具有更多的内含子和外显子，并且在所研究基因的启动子区域中通常检测到参与生物胁迫反应的顺式作用元件，如as-1、w-box和应激反应元件（STRE）。本研究的数据也支持田间受香蕉叶斑病影响的香蕉品种的表型研究。一旦在有前景的新杂交品种中得到验证，这些基因可用于标记辅助选择（MAS）和/或基因编辑技术。
