Department of Urology, Yamagata University School of Medicine, Yamagata, Japan.
Anticancer Res. 2010 Oct;30(10):4089-96.
Bacillus Calmette-Guerin (BCG) is one of therapeutic options for urothelial carcinoma (UC). The objectives of this study were to determine the direct effect of viable or heat-killed BCG and BCG cell wall skeleton (BCG-CWS) on UC cells in vitro.
UC cell lines were co-cultured with viable or heat-killed BCG Immunobladder® (Tokyo 172 strain) and BCG-CWS. Viability of the cells, apoptosis and BrdU incorporation were estimated.
BCG induced cell growth retardation in highly malignant UC bearing integrin α5β1 (VLA5). VLA5-blocking antibody partially abrogated this effect. BCG treatment induced a modest increase in the sub-G(1) fraction of cells and a decrease of BrdU incorporation. Cell growth retardation effect of viable BCG was reproduced by both heat-killed BCG and BCG-CWS.
The results indicate that VLA5 may be a biomarker of UC with sensitivity to BCG. Moreover, BCG-CWS is a promising substance which might replace BCG, preventing life-threatening complications of viable BCG treatment.
卡介苗（BCG）是治疗膀胱癌（UC）的方法之一。本研究旨在确定活的或热灭活的卡介苗和卡介苗细胞壁骨架（BCG-CWS）对体外 UC 细胞的直接作用。
将 UC 细胞系与活的或热灭活的卡介苗免疫膀胱（东京 172 株）和 BCG-CWS 共培养。评估细胞活力、细胞凋亡和 BrdU 掺入。
BCG 诱导整合素 α5β1（VLA5）高恶性 UC 细胞生长迟缓。VLA5 阻断抗体部分阻断了这种作用。BCG 处理诱导细胞亚 G1 期分数适度增加和 BrdU 掺入减少。活的 BCG 的细胞生长抑制作用可由热灭活的 BCG 和 BCG-CWS 重现。
结果表明 VLA5 可能是对 BCG 敏感的 UC 的生物标志物。此外，BCG-CWS 是一种有前途的物质，可能替代 BCG，防止活的 BCG 治疗的危及生命的并发症。
