Department of Entomology, Virginia Tech, Blacksburg, Virginia 24061, USA.
Am J Trop Med Hyg. 2010 Nov;83(5):1023-7. doi: 10.4269/ajtmh.2010.10-0366.
Physical mapping is a useful approach for studying genome organization and evolution as well as for genome sequence assembly. The availability of polytene chromosomes in malaria mosquitoes provides a unique opportunity to develop high-resolution physical maps. We report a 0.6-Mb-resolution physical map consisting of 422 DNA markers hybridized to 379 chromosomal sites of the Anopheles stephensi polytene chromosomes. This makes An. stephensi second only to Anopheles gambiae in density of a physical map among malaria mosquitoes. Three hundred sixty-three (363) probes hybridized to single chromosomal sites, whereas 59 clones yielded multiple signals. This physical map provided a suitable basis for comparative genomics, which was used for determining inversion breakpoints, duplications, and origin of novel genes across species.
物理图谱绘制是研究基因组结构和进化以及基因组序列组装的一种有用方法。多线染色体在疟原蚊中的存在为开发高分辨率物理图谱提供了独特的机会。我们报告了一个由 422 个 DNA 标记组成的 0.6Mb 分辨率的物理图谱，这些标记与 379 个按蚊斯蒂芬斯多线染色体上的染色体位点杂交。这使得按蚊斯蒂芬斯在疟原蚊中的物理图谱密度上仅次于冈比亚按蚊。363 个探针与单个染色体位点杂交，而 59 个克隆产生多个信号。该物理图谱为比较基因组学提供了一个合适的基础，用于确定跨物种的倒位断点、重复和新基因的起源。
