Barroso-Moguel R, Ríos-Castañeda C, Villeda-Hernández J, Méndez-Armenta M, Galván-Arzate S
Laboratory of Celular Neuromorphology, Instituto Nacional de Neurología Dr. Manuel Velasco Suárez, México, D.F.
Arch Invest Med (Mex). 1990 Apr-Jun;21(2):115-22.
A biochemical study of experimental thallium intoxication in newborn rats and its morphological consequences upon the brain of developing animals was undertaken. The thallium content was analyzed in the following encephalic regions: hippocampus, hypothalamus, mesencephalon, cerebellum, and cortex. One day after application of the toxic substance, a homogeneous distribution of the metal in the brain was found. Thallium concentrations in the mentioned regions were twice as high as those found in an adult rat, at the same dosage, 20 newborn Wistar rats were used for the histopathological study. 5 remained as witnesses, the rest received a single injection of thallium of .07 ml. of a solution with a concentration of .32 mg/Kg. 3 rats were sacrificed at 24, 48 and 72 hours; 3 at 7 days and 3 at 51 days. The brain , sciatic and crural nerves were fixated in 10% formaldehyde for 15 days. Cuts in paraffin and frozen sections measuring between 5 and 7 microns were taken from the fragments of different areas. These were stained with aniline methods (Masson, Gallego and H-E) and silver-gold impregnation as modified by Río-Hortega.
对新生大鼠实验性铊中毒进行了生化研究，并观察了其对发育中动物大脑的形态学影响。分析了以下脑区的铊含量：海马体、下丘脑、中脑、小脑和皮质。在施用有毒物质一天后，发现大脑中金属分布均匀。在相同剂量下，上述区域的铊浓度是成年大鼠的两倍。使用20只新生Wistar大鼠进行组织病理学研究。5只作为对照，其余大鼠单次注射0.07毫升浓度为0.32毫克/千克的铊溶液。分别在24小时、48小时和72小时处死3只大鼠；7天时处死3只；51天时处死3只。将大脑、坐骨神经和股神经在10%甲醛中固定15天。从不同区域的组织块上切取5至7微米厚的石蜡切片和冰冻切片。这些切片用苯胺方法（Masson法、Gallego法和苏木精-伊红染色法）以及经Río-Hortega改良的银金浸染法染色。
