Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Yenepoya Research Center, Yenepoya University, University Road, Deralakatee, Mangalore, Karnataka, India.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2348-2352. doi: 10.1099/ijs.0.027524-0. Epub 2010 Oct 29.
A Gram-staining-positive coccus, designated CC-SPL15-2(T), was isolated from the rhizosphere of Sesuvium portulacastrum. By 16S rRNA gene sequence analysis, it was shown that strain CC-SPL15-2(T) belonged to the genus Salinicoccus. The isolate was most closely related to Salinicoccus hispanicus DSM 5352(T) (98.3 % 16S rRNA gene sequence similarity) and Salinicoccus roseus DSM 5351(T) (96.7 %); similarities to all other members of the genus Salinicoccus were <96.5 %. In accordance with characteristics of the genus Salinicoccus, the quinone system was mainly composed of menaquinone MK-6. The polar lipid profile exhibited the major components diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. In the polyamine pattern, spermidine was the predominant compound. The fatty acids were anteiso-C(15 : 0), iso-C(15 : 0), iso-C(16 : 0) and anteiso-C(17 : 0), which supported the affiliation of strain CC-SPL15-2(T) to the genus Salinicoccus. DNA-DNA relatedness between strain CC-SPL15-2(T) and S. hispanicus CCUG 43288(T) was 42 and 32 % (reciprocal analysis). From these data as well as from physiological and biochemical tests, a clear differentiation of strain CC-SPL15-2(T) from S. hispanicus and other members of the genus Salinicoccus was possible. We propose that strain CC-SPL15-2(T) be assigned to a novel species, with the name Salinicoccus sesuvii sp. nov. The type strain is CC-SPL15-2(T) ( = DSM 23267(T) = CCM 7756(T)).
一株革兰氏阳性球菌，被命名为 CC-SPL15-2(T)，从 Sesuvium portulacastrum 的根际土壤中分离得到。通过 16S rRNA 基因序列分析，该菌株属于 Salinicoccus 属。该菌株与 Salinicoccus hispanicus DSM 5352(T)（98.3% 16S rRNA 基因序列相似性）和 Salinicoccus roseus DSM 5351(T)（96.7%）最为密切相关；与属内所有其他成员的相似性均<96.5%。根据 Salinicoccus 属的特征，醌类系统主要由menaquinone MK-6 组成。极性脂谱显示主要成分为双磷脂酰甘油、磷脂酰甘油和一种未鉴定的糖脂。在多胺图谱中，腐胺是主要化合物。脂肪酸为 anteiso-C(15:0)、iso-C(15:0)、iso-C(16:0)和 anteiso-C(17:0)，这支持菌株 CC-SPL15-2(T)归属于 Salinicoccus 属。菌株 CC-SPL15-2(T)与 S. hispanicus CCUG 43288(T)之间的 DNA-DNA 同源性为 42%和 32%（相互分析）。从这些数据以及生理生化试验结果来看，菌株 CC-SPL15-2(T)与 S. hispanicus 和 Salinicoccus 属的其他成员之间存在明显的分化。我们建议将菌株 CC-SPL15-2(T)分配到一个新的物种中，命名为 Salinicoccus sesuvii sp. nov.。其模式菌株为 CC-SPL15-2(T)（=DSM 23267(T)=CCM 7756(T)）。
