Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500 046, India.
Antonie Van Leeuwenhoek. 2013 Apr;103(4):885-98. doi: 10.1007/s10482-012-9870-4. Epub 2013 Jan 10.
Strain JC90(T) was isolated from a soda lake in Lonar, India. Strain JC90(T) maintains its external pH to 8.5 and participates in halite formation. Based on 16S rRNA gene sequence similarity studies, strain JC90(T) was found to belong to the genus Salinicoccus and is most closely related to "Salinicoccus kekensis" K164(T) (99.3 %), Salinicoccus alkaliphilus T8(T) (98.4 %) and other members of the genus Salinicoccus (<96.5 %). However Strain JC90(T) is <36 % related (based on DNA-DNA hybridization) with the type strains of "S. kekensis" K164(T) and S. alkaliphilus T8(T). The DNA G+C content of strain JC90(T) was determined to be 46 mol %. The cell-wall amino acids were identified as lysine and glycine. Polar lipids were found to include diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl ethanolamine, an unidentified glycolipid and unidentified lipids (L1,2). Major hopanoids of strain JC90(T) were determined to be bacterial hopane derivatives (BHD1,2), diplopterol, diploptene and two unidentified hopanoids (UH1,2). The predominant isoprenoid quinone was identified as menaquinone (MK-6). Anteiso-C15:0 was determined to be the predominant fatty acid and significant proportions of iso-C14:0, C14:0, iso-C15:0, C16:0, iso-C16:0, iso-C17:0, anteiso-C17:0 and C18:02OH were also detected. The results of physiological and biochemical tests support the molecular evidence and allowed a clear phenotypic differentiation of strain JC90(T) from all other members of the genus Salinicoccus. Strain JC90(T) is therefore considered to represent a novel species, for which the name Salinicoccus halitifaciens sp. nov. is proposed. The type strain is JC90(T) (=KCTC 13894(T) =DSM 25286(T)).
JC90(T) 菌株从印度 Lonar 的苏打湖中分离得到。该菌株能维持外部 pH 值在 8.5 左右，并参与石盐的形成。根据 16S rRNA 基因序列相似性研究，JC90(T) 菌株属于 Salinicoccus 属，与“Salinicoccus kekensis”K164(T)（99.3%）、Salinicoccus alkaliphilus T8(T)（98.4%）和其他 Salinicoccus 属成员最为密切相关（<96.5%）。然而，JC90(T) 菌株与“Salinicoccus kekensis”K164(T)和 S. alkaliphilus T8(T)的模式菌株的亲缘关系<36%（基于 DNA-DNA 杂交）。JC90(T) 菌株的 DNA G+C 含量为 46mol%。细胞壁氨基酸鉴定为赖氨酸和甘氨酸。极性脂包括二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的糖脂和未鉴定的脂类（L1、2）。JC90(T) 菌株的主要藿烷类化合物确定为细菌藿烷衍生物（BHD1、2）、二孢醇、二孢烯和两种未鉴定的藿烷类化合物（UH1、2）。主要的异戊二烯醌被鉴定为甲萘醌（MK-6）。anteiso-C15:0 被确定为主要脂肪酸，iso-C14:0、C14:0、iso-C15:0、C16:0、iso-C16:0、iso-C17:0、anteiso-C17:0 和 C18:02OH 的比例也很高。生理生化试验的结果支持分子证据，并使 JC90(T) 菌株与 Salinicoccus 属的所有其他成员在表型上明显分化。因此，JC90(T) 菌株被认为代表一个新种，建议命名为 Salinicoccus halitifaciens sp. nov.。模式菌株为 JC90(T)=KCTC 13894(T)=DSM 25286(T)。
