Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Finland.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2368-2372. doi: 10.1099/ijs.0.024836-0. Epub 2010 Oct 29.
This study was set up to identify three Gram-negative, rod-shaped strains originating from broiler meat packaged under a modified atmosphere. A polyphasic taxonomic approach, including multilocus sequence analysis (MLSA) of five genes (16S rRNA, glnA, gyrB, recA and HSP60), DNA-DNA reassociation between the closest phylogenetic neighbours and determination of relevant phenotypic properties, was applied. Phylogenetic analysis of the 16S rRNA gene sequences grouped these strains together and within the genus Yersinia. MLSA of the 16S rRNA gene and four housekeeping genes showed that the strains formed a monophyletic group separate from other Yersinia species in all phylogenetic trees constructed. The strains had a phenotypic profile different from those of other representatives of the genus Yersinia, but most similar to that of Yersinia ruckeri. Typical virulence markers for pathogenic Yersinia were not detected. Based on phylogenetic, phenotypic and DNA-DNA reassociation data, a novel species, Yersinia nurmii sp. nov., is proposed for the isolated strains. The type strain is APN3a-c(T) ( = DSM 22296(T) = LMG 25213(T)).
本研究旨在鉴定三种源自包装在改良气氛下的鸡肉的革兰氏阴性、杆状菌株。应用了多相分类方法，包括五个基因（16S rRNA、glnA、gyrB、recA 和 HSP60）的多位点序列分析（MLSA）、最接近的系统发育邻居之间的 DNA-DNA 重配和相关表型特性的测定。16S rRNA 基因序列的系统发育分析将这些菌株与肠杆菌属内的其他菌株聚集在一起。16S rRNA 基因和四个看家基因的 MLSA 表明，这些菌株在所有构建的系统发育树中形成了一个与其他肠杆菌属物种分开的单系群。这些菌株的表型特征与其他肠杆菌属代表菌株不同，但与鲁氏耶尔森菌最为相似。未检测到致病性耶尔森氏菌的典型毒力标记。基于系统发育、表型和 DNA-DNA 重配数据，建议将分离株命名为新种努氏耶尔森氏菌（Yersinia nurmii sp. nov.）。模式菌株为 APN3a-c（T）（ = DSM 22296（T） = LMG 25213（T））。
