Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Finland.
Finnish Food Safety Authority Evira, Hygiene Unit, Helsinki, Finland.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2363-2367. doi: 10.1099/ijs.0.019984-0. Epub 2010 Oct 29.
The taxonomic position of three strains from water, soil and lettuce samples was studied by using a polyphasic taxonomic approach. The strains were reported to lack the virulence-encoding genes inv and virF in a previous study. Controversially, API 20 E and some other phenotypic tests suggested that the strains belong to Yersinia pseudotuberculosis, which prompted this polyphasic taxonomic study. In both the phylogenetic analyses of four housekeeping genes (glnA, gyrB, recA and HSP60) and numerical analyses of HindIII and EcoRI ribopatterns, the strains formed a separate group within the genus Yersinia. Analysis of the 16S rRNA gene sequences showed that the strains were related to Yersinia aldovae and Yersinia mollaretii, but DNA-DNA hybridization analysis differentiated them from these species. Based on the results of the phylogenetic and DNA-DNA hybridization analyses, a novel species, Yersinia pekkanenii sp. nov., is proposed. The type strain is ÅYV7.1KOH2(T) ( = DSM 22769(T) = LMG 25369(T)).
采用多相分类方法研究了来自水、土壤和生菜样本的三株菌的分类地位。在之前的研究中，这些菌株被报道缺乏毒力编码基因 inv 和 virF。矛盾的是，API 20E 和其他一些表型测试表明这些菌株属于假结核耶尔森菌，这促使进行了这种多相分类研究。在四个管家基因（glnA、gyrB、recA 和 HSP60）的系统发育分析和 HindIII 和 EcoRI 核糖体图谱的数值分析中，这些菌株在耶尔森氏菌属内形成了一个单独的群体。16S rRNA 基因序列分析表明，这些菌株与 aldovae 耶尔森氏菌和 mollaretii 耶尔森氏菌有关，但 DNA-DNA 杂交分析将它们与这些物种区分开来。基于系统发育和 DNA-DNA 杂交分析的结果，提出了一个新的物种，即 pekkanenii 耶尔森氏菌。模式株为 ÅYV7.1KOH2(T)（=DSM 22769(T)=LMG 25369(T)）。
