Lehrstuhl für Mikrobielle Ökologie, Department Biowissenschaften, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany.
BMC Genomics. 2011 Mar 31;12:168. doi: 10.1186/1471-2164-12-168.
Yersinia enterocolitica strains responsible for mild gastroenteritis in humans are very diverse with respect to their metabolic and virulence properties. Strain W22703 (biotype 2, serotype O:9) was recently identified to possess nematocidal and insecticidal activity. To better understand the relationship between pathogenicity towards insects and humans, we compared the W22703 genome with that of the highly pathogenic strain 8081 (biotype1B; serotype O:8), the only Y. enterocolitica strain sequenced so far.
We used whole-genome shotgun data to assemble, annotate and analyse the sequence of strain W22703. Numerous factors assumed to contribute to enteric survival and pathogenesis, among them osmoregulated periplasmic glucan, hydrogenases, cobalamin-dependent pathways, iron uptake systems and the Yersinia genome island 1 (YGI-1) involved in tight adherence were identified to be common to the 8081 and W22703 genomes. However, sets of ~550 genes revealed to be specific for each of them in comparison to the other strain. The plasticity zone (PZ) of 142 kb in the W22703 genome carries an ancient flagellar cluster Flg-2 of ~40 kb, but it lacks the pathogenicity island YAPI(Ye), the secretion system ysa and yts1, and other virulence determinants of the 8081 PZ. Its composition underlines the prominent variability of this genome region and demonstrates its contribution to the higher pathogenicity of biotype 1B strains with respect to W22703. A novel type three secretion system of mosaic structure was found in the genome of W22703 that is absent in the sequenced strains of the human pathogenic Yersinia species, but conserved in the genomes of the apathogenic species. We identified several regions of differences in W22703 that mainly code for transporters, regulators, metabolic pathways, and defence factors.
The W22703 sequence analysis revealed a genome composition distinct from other pathogenic Yersinia enterocolitica strains, thus contributing novel data to the Y. enterocolitica pan-genome. This study also sheds further light on the strategies of this pathogen to cope with its environments.
导致人类轻度肠胃炎的小肠结肠炎耶尔森氏菌菌株在代谢和毒力特性方面具有很大的多样性。菌株 W22703（生物型 2，血清型 O:9）最近被鉴定具有杀线虫和杀虫活性。为了更好地了解昆虫致病性与人类之间的关系，我们将 W22703 基因组与高致病性菌株 8081（生物型 1B；血清型 O:8）进行了比较，这是迄今为止测序的唯一耶尔森氏菌菌株。
我们使用全基因组鸟枪法数据组装、注释和分析了 W22703 菌株的序列。许多被认为有助于肠道生存和发病机制的因素，包括渗透压调节周质葡聚糖、氢化酶、钴胺素依赖途径、铁摄取系统和紧密粘附相关的耶尔森氏菌基因组岛 1（YGI-1），在 8081 和 W22703 基因组中被发现是共同的。然而，与另一个菌株相比，每个菌株的约 550 个基因被证明是特定的。W22703 基因组中的 142kb 可塑性区（PZ）携带一个约 40kb 的古老鞭毛簇 Flg-2，但它缺乏致病性岛 YAPI（Ye）、分泌系统 ysa 和 yts1 以及 8081 PZ 的其他毒力决定因素。其组成突出了该基因组区域的显著可变性，并证明了其对生物型 1B 菌株相对于 W22703 的更高致病性的贡献。在 W22703 的基因组中发现了一种新型的镶嵌结构的 III 型分泌系统，该系统在已测序的人类致病性耶尔森氏菌菌株中不存在，但在无致病性物种的基因组中保守。我们在 W22703 中鉴定了几个差异区域，这些区域主要编码转运蛋白、调节剂、代谢途径和防御因子。
W22703 序列分析揭示了与其他致病性小肠结肠炎耶尔森氏菌菌株不同的基因组组成，从而为小肠结肠炎耶尔森氏菌全基因组提供了新的数据。本研究还进一步揭示了该病原体应对其环境的策略。
