Max-Planck-Institut für Infektionsbiologie, Department of Molecular Biology, Berlin, Germany.
Nat Genet. 2010 Dec;42(12):1140-3. doi: 10.1038/ng.705. Epub 2010 Oct 31.
Plague is a pandemic human invasive disease caused by the bacterial agent Yersinia pestis. We here report a comparison of 17 whole genomes of Y. pestis isolates from global sources. We also screened a global collection of 286 Y. pestis isolates for 933 SNPs using Sequenom MassArray SNP typing. We conducted phylogenetic analyses on this sequence variation dataset, assigned isolates to populations based on maximum parsimony and, from these results, made inferences regarding historical transmission routes. Our phylogenetic analysis suggests that Y. pestis evolved in or near China and spread through multiple radiations to Europe, South America, Africa and Southeast Asia, leading to country-specific lineages that can be traced by lineage-specific SNPs. All 626 current isolates from the United States reflect one radiation, and 82 isolates from Madagascar represent a second radiation. Subsequent local microevolution of Y. pestis is marked by sequential, geographically specific SNPs.
鼠疫是一种由鼠疫耶尔森菌引起的、具有全球流行趋势的人类侵袭性疾病。本研究报告了全球来源的 17 株鼠疫耶尔森菌全基因组的比较。我们还利用 Sequenom MassArray SNP 分型技术，对来自全球的 286 株鼠疫耶尔森菌分离株进行了 933 个单核苷酸多态性的筛选。我们对这组序列变异数据集进行了系统发育分析，基于最大简约法将分离株分配到不同种群中，并根据这些结果推断了历史传播途径。我们的系统发育分析表明，鼠疫耶尔森菌起源于中国或其附近地区，并通过多次辐射传播到欧洲、南美洲、非洲和东南亚，导致形成了具有国家特异性的谱系，这些谱系可以通过谱系特异性单核苷酸多态性来追踪。来自美国的 626 株当前分离株都反映出一次辐射，来自马达加斯加的 82 株分离株代表了第二次辐射。随后鼠疫耶尔森菌的局部微进化以连续的、具有地理特异性的单核苷酸多态性为标志。
